摘要
为筛选并确定能够显著提高大豆抗盐性的海藻多糖种类,本研究以大豆品种中黄13为供试材料,在大豆两片真叶完全展开后,用100 mmol·L^(-1)NaCl溶液进行盐胁迫处理,通过对大豆叶片喷施0.1%的海藻多糖溶液,比较分析不同种类海藻多糖对盐胁迫下大豆幼苗生长状况和生理指标的影响。结果表明:盐胁迫下,大豆幼苗遭受渗透胁迫和氧化胁迫,生长受到抑制。施用不同种类的海藻多糖能够不同程度缓解盐胁迫对大豆幼苗生长的抑制。相较于盐胁迫组,喷施岩藻多糖LT使大豆幼苗株高提高17.86%,根长提高12.37%,根干重提高35.71%;施用不同种类的海藻多糖还能够不同程度增加叶绿素和渗透调节物质含量,显著提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化酶活性,降低丙二醛(MDA)含量,提高质膜保护能力。综上,不同种类海藻多糖均能提高大豆幼苗的抗盐能力,其中岩藻多糖比褐藻酸钠和浒苔多糖对大豆幼苗的抗盐性的促进作用更强。
In order to screen the types of seaweed polysaccharides that can significantly improve the salt resistance of soybeans.The soybean variety Zhonghuang 13 were identified with 100 mmol·L^(-1)NaCl solution for screening tolerant soybean varieties in soybean seedling stage.After the two true leaves of soybeans have fully unfolded,spray 0.1%of the seaweed polysaccharide solution to compare the effects of different types of seaweed polysaccharides on the growth and physiological indicators of soybean seedlings under salt stress.The results showed that under salt stress,soybean seedlings were suffering from penetration stress and oxidation stress,and growth was suppressed.Applying different types of seaweed polysaccharides can alleviate the inhibition of salt stress on soybean seedling growth to varying degrees,among them spraying fucoidan LT increased the height of plants by 17.86%,root length by 12.37%,and root dry weight by 35.71%compared to the salt stress group.In addition,applying different types of seaweed polysaccharides can also increase chlorophyll and penetrate the material content,significantly enhance the superoxide dismutase(SOD),excessive Oxidase(POD)and Catalase(CAT)activity.The application of different seaweed polysaccharides can also reduce the content of malondialdehyde(MDA)content in soybean seedlings and improve the protective ability of plasma membrane.In conclusion,different types of seaweed polysaccharides can improve the salt resistance of soybean seedlings.Among them,fucoidans was more effective than sodium alginate and polysaccharide from Enteromorpha prolifera on soybean seedling salt stress resistance.
作者
邹平
杨霞
于喆妍
马斯琦
荆常亮
李义强
ZOU Ping;YANG Xia;YU Zheyan;MA Siqi;JING Changliang;LI Yiqiang(Institute of Tobacco Research of CAAS,Qingdao 266101,China;National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land,Dongying 257300,China;College of Agronomy,Qingdao Agricultural University,Qingdao 266109,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2024年第1期73-80,共8页
Soybean Science
基金
中央级公益性科研院所基本科研业务费项目(1610232023009)。
关键词
大豆
盐胁迫
海藻多糖
岩藻多糖
soybean
salt stress
seaweed polysaccharide
fucoidan
