摘要
【目的】探索盐胁迫对杠柳幼苗生长及次生代谢物的影响,初步了解杠柳盐耐受范围及盐胁迫对杠柳次生代谢产物的诱导作用。【方法】以培养30 d的杠柳实生苗为材料,测定不同盐浓度[0(CK)、0.2%、0.5%、1.0%、2.0%]处理后,杠柳幼苗的生物量及总甾体成分、强心苷类成分(杠柳毒苷、杠柳次苷及杠柳苷元)、4-甲氧基水杨醛含量的变化特征。【结果】杠柳幼苗在中低浓度盐胁迫下生长良好,且地上部分干鲜质量及地下部分干质量均有不同程度增加,其干湿比也均随盐胁迫程度的增加逐渐升高;植株体内的主要有效成分如总甾体、强心苷类的含量在中等盐胁迫浓度作用下呈现明显上升趋势,而4-甲氧基水杨醛含量则在高浓度盐胁迫下显著增加。【结论】杠柳具有较强的耐盐性,且一定浓度范围内的盐胁迫可显著提高杠柳幼苗根、茎中主要药效成分含量。
[Objective]The study aims to explore the effects of salt stress on the growth and secondary metabolites of Periploca sepium seedlings,and preliminarily understand the salt tolerance range of P.sepium and the induction effect of salt stress on secondary metabolites of P.sepium.[Methods]This experiment was conducted with the seedlings of P.sepium cultivated for 30 days to determine the changes in biomass,total steroids components,cardiac glycosides(periplocin,periplocymarin,and periplogenin),and 4-methoxy salicylaldehyde content of P.sepium seedlings treated with different salt concentrations(0,0.2%,0.5%,1.0%,2.0%).[Results]The P.sepium seedlings grew well under low concentration of salt stress,and the dry and fresh biomass of the aboveground and underground parts were increased to varying degrees.The dry to fresh ratio was gradually increased with the increase in salt stress levels.The content of the major effective components in plants,such as total steroids and cardiac glycosides,showed a significant upward trend under moderate salt stress,while 4-methoxy salicylaldehyde was significantly increased under high salt stress.[Conclusion]P.sepium has strong salt tolerance,and salt stress within a certain range increases the content of major secondary metabolites in the roots and stems of P.sepium seedlings.
作者
韩翠婷
李先宽
王广苹
马琳
张坚
HAN Cuiting;LI Xiankuan;WANG Guangping;MA Lin;ZHANG Jian(School of Traditional Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第2期280-287,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
天津市道地药材生态种植及质量保障项目(2022)。
关键词
杠柳
盐胁迫
生物量
次生代谢产物
Periploca sepium Bunge
salt stress
biomass
secondary metabolites
