摘要
为明晰朱砂梅品种群梅花花色表型差异形成的分子调控机制,以花色由浅至深呈梯度变化的6个朱砂梅品种为试验材料,利用色差仪和分光光度计测定不同品种盛花期花瓣的花色表型及花色苷含量,通过荧光定量PCR及相关性分析,对花色苷合成结构基因(PmCHS、PmCHI、PmF3H、PmF3′H、PmDFR、PmANS、PmUFGT)及转录因子编码基因(PmMYB1、PmbHLH3)的转录特性进行探究。结果表明,各品种间花瓣花色苷含量与花色红度的变化趋势一致;PmMYB1、PmbHLH3、PmF3′H、PmDFR、PmUFGT的相对表达量与花色苷含量呈极显著正相关;转录因子编码基因PmMYB1与PmbHLH3的相对表达量呈极显著正相关,且均与结构基因PmF3′H、PmDFR、PmUFGT的表达水平呈显著或极显著正相关。推测转录因子编码基因PmMYB1PmbHLH3通过正向调控结构基因PmF3′H、PmDFR和PmUFGT的表达从而调控朱砂梅花瓣的呈色。
To clarify the molecular regulatory mechanism for the formation of phenotypic differences in flower color of Prunus mume Cinnabar Purple Group,six varieties of P.mume Cinnabar Purple Group with gradient changes in flower color from light to dark were used as the test materials.The floral color phenotypes and anthocyanin contents of petals of different varieties at full-bloom stage were determined by colorimeter and spectrophotometer,respectively.The transcriptional characteristics of the structural genes for anthocyanin synthesis(PmCHS,PmCHI,PmF3H,PmF3′H,PmDFR,PmANS,PmUFGT)and the transcription factors encoding genes(PmMYB1,PmbHLH3)were analyzed by qRT-PCR and correlation analysis.The results showed that the change trend of anthocyanin content in petals among the varieties was consistent with that of flower color redness.The relative expression of PmMYB1,PmbHLH3,PmF3′H,PmDFR and PmUFGT were highly significantly and positively correlated with anthocyanin content.The relative expression of transcription factors encoding genes PmMYB1 and PmbHLH3 were highly significantly and positively correlated,and all of them were significantly or highly significantly and positively correlated with the expression levels of the structural genes PmF3′H,PmDFR and PmUFGT.It is hypothesized that the transcription factors encoding genes PmMYB1 and PmbHLH3 regulate the expression of the structural genes PmF3′H,PmDFR and PmUFGT and thus regulate the color presentation of the petals of P.mume Cinnabar Purple Group.
作者
郭梦鸽
秦孝天
陈瑞丹
GUO Meng-ge;QIN Xiao-tian;CHEN Rui-dan(School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,Beijing 100083,China;National Engineering Research Center for Floriculture,Beijing 100083,China;Beijing Laboratory of Urban and Rural Ecological Environment,Beijing 100083,China;Engineering Research Center of Landscape Environment of Ministry of Education,Beijing 100083,China;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing 100083,China)
出处
《江苏农业学报》
CSCD
北大核心
2024年第2期367-375,共9页
Jiangsu Journal of Agricultural Sciences
基金
北京园林绿化增彩延绿科技创新工程项目(2019KJC0210)。
