摘要
为研究茶叶酚类成分及其复方对P.aeruginosa PAO1的体外抑制作用,筛选出用药量最低的最佳抑菌组方,采用倍比稀释法结合刃天青显色测定5种酚类成分对P.aeruginosa PAO1的最小抑菌浓度(MIC),并采用响应面法研究对P.aeruginosa PAO1具有较强抑制作用的最佳配伍。结果表明5种酚类成分对P.aeruginosa PAO1均有不同程度的抑制作用,5种茶叶活性成分对P.aeruginosa PAO1的抑菌性大小为:EGCG>TF60=ECG>EGC>EC。响应面分析结果表明,EGCG、EGC、ECG、TF60这4种酚类成分以1.0∶4.0∶4.81∶4.0的比例配伍时对P.aeruginosa PAO1具有最佳抑菌效果。
To study the in vitro inhibitory effects of tea phenolic components and their combinations on P.aeruginosa PAO1,and the best antibacterial prescription with the lowest dosage was screened.The minimum inhibitory concentration(MIC)of five phenolic components on P.aeruginosa PAO1 was determined by multiple ratio dilution method combined with the color rendering of resazurin,and the response surface method was used to study the optimal compatibility with strong inhibitory effect on P.aeruginosa PAO1.The results showed that all five phenolic components had different degrees of inhibitory effects on P.aeruginosa PAO1.The antibacterial activity of the five tea active components on P.aeruginosa PAO1 was EGCG>TF60=ECG>EGC>EC.The results of response surface analysis showed that EGCG,EGC,ECG and TF60 had the best bacteriostatic effect on P.aeruginosa PAO1 when they were combined in the ratio of 1.0:4:4.81:4.0.Conclusion:The combination of EGCG,EGC,ECG and TF60 has obvious inhibitory effect on P.aeruginosa PAO1.
作者
刁春华
杨秀芳
涂云飞
宋鹏鹏
左小博
苏小琴
孔俊豪
DIAO Chunhua;YANG Xiufang;TU Yunfei;SONG Pengpeng;ZUO Xiaobo;SU Xiaoqin;KONG Junhao(Hangzhou Tea Research Institute,CHINA COOP/Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resources,Hangzhou 310016,China)
出处
《茶叶通讯》
2024年第1期85-90,共6页
Journal of Tea Communication
基金
国家重点研发计划资助(2022YFD2101103)
浙江省基础公益研究计划项目(LGN19C160001)。
关键词
茶叶
酚类成分
铜绿假单胞菌
抑菌活性
Tea
Phenolic components
Pseudomonas aeruginosa
Antimicrobial activity
