摘要
目的:研究龙胆苦苷对果糖诱导小鼠高尿酸血症的治疗作用。方法:昆明种小鼠连续饮用10%果糖溶液12周以制备高尿酸症小鼠模型,从第9周开始,别嘌呤醇组,龙胆苦苷高、中、低剂量组(80 mg/kg、40 mg/kg、20 mg/kg-)每天灌胃给药1次,共给药4周。末次给药后取血,检测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、尿酸(UA)、肌酐(Cr)和血尿素氮(BUN)水平,苏木精-伊红(HE)染色观察肝、肾组织的变化;酶联免疫吸附试验法检测肾组织中超氧化物歧化酶(SOD),丙二醛(MDA),肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)水平,比色法和蛋白质免疫印迹法检测肝组织中黄嘌呤氧化酶(XOD)活性及蛋白表达水平。结果:与模型组比较,龙胆苦苷各给药组小鼠血清GPT、GOT、UA、Cra和BUN水平显著降低(P<0.01),肝、肾病理学变化有所改善;肾脏SOD水平显著升高(P<0.01),MDA、TNF-α和IL-6水平显著降低(P<0.01);同时肝脏XOD的活性及蛋白表达水平显著下调(P<0.01或P<0.05)。结论:龙胆苦苷通过降低XOD活性和表达,降低尿酸水平,进而减轻肾脏氧化应激和炎症水平,达到肾脏保护作用。
Objective:To investigate the effect of gentiopicroside on fructose-induced hyperuricemia in mice.Methods:The hyperuricmia KM mice were induced by feeding water containing 10%fructose for consecutive 12 weeks.During the period from 9th week to 12th week,mice from allopurinol group and gentiopicroside groups(80,40,20 mg/kg)received allopurinol and gentiopicroside,respectively by intragastric administration once a day for 4 weeks.The blood was collected after the last administration,to detect glutamic pyruvic transaminase(GPT),glutamic oxaloacetic transaminase(GOT),uric acid(UA),creatinine(Cr)and blood urea nitrogen(BUN)in serum.H&E staining was performed to observe the changes in liver and kidney tissues.Superoxide dismutase(SOD),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)in kidney tissues were measured by enzyme-linked immunosorbent assay(ELISA),and the activity and expression of XOD in liver were tested by colorimetry and Western Blotting.Results:Compared with the model group,the mice in gentiopicroside groups showed a decrease in serum GPT,GOT,UA,Cr and BUN(P<0.01),with improvement in pathological changes of liver and kidney.The SOD in kidney were increased,whereas MDA,TNF-αand IL-6 were lowered(P<0.01).Moreover,gentiopicroside reduced the activity and protein expression of XOD in liver(P<0.01 or P<0.05).Conclusion:Gentiopicroside down-regulates UA in the fructose-induced hyperuricemia mice by modulating the activity and the expression of XOD,thereby alleviating oxidative stress,and inflammation and protecting the kidney.
作者
李静
尚平平
杨洋
何姣
乔博灵
LI Jing;SHAGN Pingping;YANG Yang;HE Jiao;Qiao Boling(Biomedicine Key Laboratory of Shaanxi Province,College of Life Science,Northwest University,Xi′an 710016,China;Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China,College of Life Sciences,Shaanxi Normal University,Xi′an 710119,China)
出处
《世界中医药》
CAS
北大核心
2024年第1期26-30,共5页
World Chinese Medicine
基金
国家自然科学基金项目(82003928)——基于ERS-UPR跨膜效应蛋白研究秦艽治疗NAFLD的有效物质基础及作用机制
陕西省教育厅专项科研计划项目(2013JK0809)——龙胆苦苷抗高尿酸血症的作用机制研究
陕西省教育厅省级重点实验室科研计划项目(11JS089)——玄参黑度的研究。
关键词
龙胆苦苷
果糖
高尿酸血症
尿酸
黄嘌呤氧化酶
氧化应激
炎症
肾脏保护
Gentiopicroside
Fructose
Hyperuricemia
Uric acid
Xanthine oxidase
Oxidative stress
Inflammation
Kidney protection
