摘要
目的:建立一种生活饮用水中乙二胺四乙酸(EDTA)和次氮基三乙酸(NTA)的高效液相色谱测定方法。方法:样品与三氯化铁络合后,以甲醇和0.2 mmol/L四丁基溴化铵—磷酸缓冲溶液(pH 2.5)为流动相,梯度洗脱后经Thermo-C_(18)(4.6 mm×250 mm)色谱柱分离,DAD检测器检测,外标法定量。结果:EDTA和NTA在0.1~2.0 mg/L质量浓度范围内与色谱峰面积线性关系良好,相关系数分别为0.9995,0.9992;两者的检出限为0.05 mg/L,定量限为0.1 mg/L,平均加标回收率为93.5%~111.4%,相对标准偏差(RSD)为3.63%~7.39%。结论:该方法操作简便,重复性好,灵敏度高,具有较好的实用性。
Objective:A high performance liquid chromatography method was developed to determine EDTA and NTA in water.Methods:The samples were derivatized with ferric chloride,used methanol and a 0.2 mmol/L tetrabutylammonium bromide phosphate buffer solution(pH 2.5)as the mobile phase,separated on a Thermo-C_(18)(4.6 mm×250 mm)column after gradient elution,detected by HPLC with a diode array detector,and used external standard method for quantitation.Results:The content of EDTA and NTA showed a good linear relationship in the range of 0.1~2.0 mg/L,the correlation coefficients were 0.9995 and 0.9992 respectively;The limits of detection were 0.05 mg/L and limits of quantification were 0.1 mg/L;The average recoveries were 93.5%~111.4%;And the relative standard deviations(RSD)were 3.63%~7.39%.Conclusion:This method was convenient,repeatable,sensitive,and practical for the detection of EDTA and NTA.
作者
王雪婷
郑斌
WANG Xueting;ZHENG Bin(CAIQ〔Shanghai〕Testing Technology Co.,Ltd.,Shanghai 201206,China)
出处
《食品与机械》
CSCD
北大核心
2024年第2期74-77,232,共5页
Food and Machinery
