摘要
目的 观察益肾活血通窍法对D-半乳糖腹腔注射致老化大鼠模型前庭内侧核磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(AKT)信号通路和氧化应激表达水平的影响,探讨益肾活血通窍法延缓前庭内侧核老化的机制。方法 18只大鼠适应性饲养7 d后随机分成空白组、模型组和中药组,各6只。空白组腹腔注射生理盐水500 mg/(kg·d),模型组、中药组腹腔注射D-半乳糖500 mg/(kg·d),连续注射8周。造模成功后,空白组、模型组灌服生理盐水18.45 g/(kg·d),中药组灌服益肾活血通窍方18.45 g/(kg·d),连续灌服8周。末次给药后2 h评估各组大鼠前庭功能,然后进行前庭内侧核取材,通过ELISA法检测各组血清谷胱甘肽(GSH)、丙二醛(MDA)、活性氧(ROS)的表达水平,通过Westernblot法检测各组前庭内侧核PI3K、AKT1的表达水平。结果 三组空中翻正反射实验成功率比较,差异无统计学意义(P>0.05)。模型组、中药组的头偏斜角度大于空白组,中药组的头偏斜角度小于模型组,差异均有统计学意义(P<0.05)。与空白组比较,模型组血清GSH的表达水平降低,MDA、ROS的表达水平增高,前庭内侧核组织中PI3K、AKT1表达水平降低,差异均有统计学意义(P<0.05)。与模型组比较,中药组血清GSH表达水平及前庭内侧核中PI3K、AKT1表达水平升高,血清MDA、ROS的表达水平降低,差异均有统计学意义(P<0.05)。结论 益肾活血通窍法对老化大鼠模型前庭内侧核具有保护作用,其机制可能与调控PI3K/AKT信号通路与氧化应激相关。
Objective To observe the effects of Yishen Huoxue Tongqiao method on the PI3K/AKT signaling pathway and oxidative stress expression level in the medial vestibular nucleus of aging model rats induced by intraperitoneal injection of D-galactose,and to explore the mechanism of Yishen Huoxue Tongqiao method in delaying the aging of the medial vestibular nucleus.Methods After adaptive feeding of 18 rats for 7 days,they were randomly divided into a blank group,a model group,and a Traditional Chinese Medicine group,with 6 rats in each group.The blank group was intraperitoneally injected with 500 mg/(kg·d)of physiological saline,while the model group and Traditional Chinese Medicine group were intraperitoneally injected with 500 mg/(kg·d)of D-galactose for 8 consecutive weeks.After successful modeling,the blank group and model group were orally administered physiological saline at 18.45 g/(kg·d),while the Traditional Chinese Medicine group was orally administered Yishen Huoxue Tongqiao decoction at 18.45 g/(kg·d)for 8 consecutive weeks.The vestibular function of each group of rats 2 hours after the last administration was evaluated,and the samples from the medial vestibular nucleus were collected.The expression levels of glutathione(GSH),malondialdehyde(MDA),reactive oxygen species(ROS)in each group were detected by the Elisa method,and the expression levels of PI3K and AKT1 in the medial vestibular nucleus were detected by the Western blot method.Results There was no statistically significant difference in the success rates of three sets of aerial righting reflex tests(P>0.05).The head deviation angle of the model group and the Traditional Chinese Medicine group was greater than that of the blank group,while the head deviation angle of the Traditional Chinese Medicine group was smaller than that of the model group,and the differences were statistically significant(P<0.05).Compared with the blank group,the expression of serum GSH in the model group decreased,while the expression of MDA and ROS increased.The expression levels of PI3K and AKT1 in the medial vestibular nucleus tissue decreased,and the differences were statistically significant(P<0.05).Compared with the model group,the expression levels of serum GSH and PI3K and AKT1 in the medial vestibular nucleus were increased in the Traditional Chinese Medicine group,while the expression levels of serum MDA and ROS were reduced,with statistical significance(P<0.05).Conclusion The Yishen Huoxue Tongqiao method has a protective effect on the medial vestibular nucleus of aging model rats,and its mechanism may be related to regulating the PI3K/AKT signaling pathway and oxidative stress.
作者
张琦
冷辉
金婧
孙嘉蔚
田雨
Zhang Qi;Leng Hui;Jin Jing;Sun Jiawei;Tian Yu(Department of ENT&HN,Liaoning University of Traditional Chinese Medicine Affiliated Hospital,Shenyang 110000;Graduate School,Liaoning University of Traditional Chinese Medicine,Liaoning 110847)
出处
《国际老年医学杂志》
2024年第2期162-166,共5页
International Journal of Geriatrics
基金
辽宁省应用基础研究计划项目(2023JH2/101300055)
吴阶平基金(320.6750.18332)
辽宁中医药大学附属医院“育苗工程”项目(YM202030)。
