摘要
为从分子水平分析胡椒鲷属鱼类分子系统分类关系,澄清物种分类争议,采集印度-西太平洋分布的胡椒鲷鱼类16种,利用PCR扩增及测序方法获得这16种鱼类线粒体Cyt b及核RAG2、S7标记序列,基于最大似然法与贝叶斯法构建分子系统进化关系。结果显示:16种鱼类Cyt b同源序列为657 bp,编码219个氨基酸;RAG2同源序列为726 bp,编码243个氨基酸;S7基因同源序列为729 bp,序列存在大量碱基插入与缺失。以大斑石鲈及断斑石鲈为外类群构建分子系统进化树,进化树上胡椒鲷鱼类主要分为3个分支,分支Ⅰ胡椒鲷具鲜艳的体色与斑纹,分支Ⅱ与分支Ⅲ胡椒鲷色彩单一,无鲜艳斑纹,与形态分类结果相似。少棘胡椒鲷属在进化树上并未形成独立的分支,而是位于胡椒鲷属内部。比较分析少棘胡椒鲷属、胡椒鲷属鱼类鳍棘数目与各物种在进化树上的分类关系,并未发现鳍棘数目与物种亲缘关系存在相关性。研究结果表明,在胡椒鲷类群中,背鳍鳍棘数可作为形态上区分不同胡椒鲷种类的指标,但未必能作为胡椒鲷鱼类亲缘关系远近的划分依据。结果支持目前大多数分子分类研究结果,少棘胡椒鲷鱼类应归为胡椒鲷属。
A total of 16 species of sweetlips were obtained,14 species belonged to the genus Plectorhinchus,2 species belonged to the genus Diagramma,domestic samples from the South China Sea and surrounding seas,while most of the foreign samples from the Indian Ocean,one in the South Pacific,and the mitochondrial Cyt b and nuclear RAG2 and S7 labeling sequences of these 16 fish species were obtained by PCR amplification and sequencing.The phylogenetic relationship of analytical molecules was constructed based on maximum likelihood method and Bayesian method.The results showed that the homologous sequence of 16 sweetlips in Cyt b genes were determined to be 657 bp,encoding 219 amino acids;the homologous sequences in RAG2 gene were 726 bp,encoding 243 amino acids,the homologous sequence in S7 genes were 729 bp,with numerous base insertions and deletions.The molecular phylogenetic tree were constructed using Pomadasys maculatus and P.hasta as outgroup species,sweetlips in the tree were divided into three major groups.Species in groupⅠpossessed spectacular coloration and markings along bodies while species in groupⅡand groupⅢhad monotone color and uniformly dark patterns,which were consistent with the morphological classification.Genus Diagramma did not form an independent branch but were clustered inside the Plectorhinchus group.Comparison of the dorsal spine numbers of Diagramma and Plectorhinchus with their relationships in the phylogenetic tree revealed that no correlation was found between dorsal spine numbers and species phylogenetic relationship,indicating that numbers of dorsal spines was used as an indicator to distinguish different sweetlips morphologically,but not be used as a basis to investigate the their relationships.The findings supported the viewpoints of most current molecular phylogenetic studies that genus Diagramma was classified into genus Plectorhinchus.
作者
程静
张癸新
陈厚桦
杨森
李培源
陈静仪
梁日深
CHENG Jing;ZHANG Guixin;CHEN Houhua;YANG Sen;LI Peiyuan;CHEN Jingyi;LIANG Rishen(Shanghai Vocational College of Agriculture and Forestry,Shanghai 201600,China;College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
出处
《水产科学》
CAS
CSCD
北大核心
2024年第2期234-242,共9页
Fisheries Science
基金
国家自然科学基金资助项目(31802300)
广东省自然科学基金资助项目(2018A0303130104)
广东省大学生创新创业训练计划项目(201911347009,202011347047)。
