摘要
铁调节转运蛋白(iron-regulated transporter 1, IRT1)是植物从土壤中吸收铁的主要根系转运蛋白,参与植物铁和锌营养吸收过程,也是镉等有毒金属进入植物体内的主要途径。克隆富铁水稻(Oryza sativa L.)中IRT1基因,为研究该基因在植物铁转运过程中的功能提供参考。本研究采用同源克隆法,以富铁水稻品种‘宜香优2115’叶片总RNA为模板,通过RT-PCR获得水稻‘宜香优2115’IRT1基因,命名为OsIRT1-YXY2115,并对其进行生物信息学分析。生物信息分析表明,该基因cDNA编码区长为1 125 bp,编码374个氨基酸,蛋白序列含有9个跨膜结构,编码蛋白分子量为39.06 kD,理论等电点为8.89,溶液中的不稳定指数为45.73,为不稳定蛋白,该蛋白属于锌铁转运蛋白(ZRT/IRT-like protein, Zip)家族成员。序列同源性分析表明,其与已报道的水稻OsIRT1基因(AB070226.1)相似性最高。亚细胞定位预测分析表明,IRT1-YXY2115蛋白定位于细胞质膜中。IRT1-YXY2115基因克隆及其结构、性质与功能的初步分析为OsIRT1基因功能研究及水稻缺铁胁迫分子机理的解析提供参考和理论依据。
Iron-regulated transporter 1(IRT1)isthe main root transporter for plant to absorb iron from soil,it is also the main wayfor toxic metals such as cadmium to enter plants.The IRT1 gene of iron-rich rice(Oryza sativa L.)was cloned to provide reference for studying the function of the gene in the process of iron transportation in plants.In this study,the IRT1 cDNA sequence amplified from'Yixiangyou 2115'rice plant by reverse transcription PCR(RT-PCR),and designated as OsIRT1-YXY2115.Sequence analysis revealed that the coding region cDNA of OsIRT1-YXY2115 was 1125 bp,which encoding 374 amino acids,and it had 9 membrane-spanning domains,with a predicted molecular mass of 39.06 kD,a theoretical isoelectric point(pI)of 8.89,and the instability index in the solution was 45.73,it is an unstable protein belonging to the zinc iron transporter(ZRT/IRT-like protein,Zip)su-perfamily.The OsIRT1-YXY2115 amino acids showed high identity with the reported OsIRT1 gene of rice(AB070226.1)by NCBI homology comparison analysis.The sub-cellular localization of this protein was predicted to be the plasma membrane.The cloning of the OsIRT1-YXY2115 gene and preliminarily predicting its structure,nature and functions,provide a reference and theoretical basis for studying the biological function of the OsIRT1 gene and the molecular mechanisms underlying iron deficiency responses in rice.
作者
王建伟
贺晓岚
汤宏
顾太波
Wang Jianwei;He Xiaolan;Tang Hong;Gu Taibo(School of Science,Kaili University,Kaili,556011;School of Life and Health Science,Kaili University,Kaili,556011)
出处
《分子植物育种》
CAS
北大核心
2024年第6期1751-1758,共8页
Molecular Plant Breeding
基金
凯里学院博士专项(BS201506)
贵州省科技计划项目([2017]1167)
贵州省科技支撑计划项目([2017]2522)共同资助。
