摘要
为探讨桃响应葡萄座腔菌(Botryosphaeria dothidea)侵染的分子机制,本研究以‘丰光’油桃作为研究材料,采用高通量测序技术对被B. dothidea侵染的桃枝干组织进行转录组测序,共筛选到1 463个差异表达基因(DEGs),其中上调911个,下调552个。差异表达基因注释到GO、KEGG数据库中的数目分别为1 231、255。GO功能分类结果显示DEGs主要富集在细胞组成、分子功能和生物学过程三个方面。细胞组成主要包括细胞组分、细胞、细胞器、膜等;分子功能主要集中于催化活性、核酸结合转录因子活性、转运活性、受体活性等;生物学过程主要富集在细胞过程、代谢过程、应激反应、生物调节等。KEGG分析结果表明差异表达基因涉及66个代谢途径,且主要富集在植物与病原互作、植物激素信号转导代谢通路。大量与植物抗病性、乙烯信号途径、钙信号途径相关的基因被B. dothidea侵染所激发。随机选取16个基因进行RT-qPCR定量分析,发现RT-qPCR结果与转录组测序结果趋势一致。本研究结果明确了桃响应B. dothidea侵染过程中相关基因的表达情况,为深入研究病原物的致病机理提供参考依据。
In order to explore the molecular mechanism of peach in response to the infection of Botryosphaeria dothidea,in this study,high-throughput sequencing was used for transcriptomic sequencing of'Fengguang'peach shoot inoculated with B.dothidea.Compared with the control,1463 differentially expressed genes(DEGs)were screened,among which 911 were up-regulated and 552 were down-regulated.A total of 1231 and 255 DEGs were annotated to GO and KEGG database,respectively.GO enrichment analysis showed that DEGs were mainly enriched in cell component,molecular function and biological process.Cellular component mainly includes:cell part,cell,organelle,membrane,etc.;molecular function mainly includes:catalytic activity,nucleic acid binding transcription factor activity,transporter activity,receptor activity,etc.;biological process mainly includes:cellular process,metabolic process,response to stimulus,biological regulation,etc..KEGG enrichment analysis revealed that DEGs were involved in 66 metabolic pathways.The most significant pathways were plant-pathogen interaction and plant hormone signal transduction.A series of genes related to plant resistance,ethylene and calcium signaling way were identified from the peach shoots infected by B.dothidea.Sixteen genes were randomly selected for real-time quantitative PCR(RT-qPCR)analysis,and the results were almost consistent with that of transcriptome sequencing.These results revealed the gene expression pattern of peach in response to B.dothidea infection,provided a theoretical reference for exploring the pathogenicity mechanism of the pathogen.
作者
刘勇
王富荣
王会良
艾小艳
朱炜
孙中海
吕亮
龚林忠
何华平
Liu Yong;Wang Furong;Wang Huiliang;Ai Xiaoyan;Zhu Wei;Sun Zhonghai;Li Liang;Gong Linzhong;He Huaping(Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences,Wuhan,430064;Institute of Plant Protection and Soil Fertility,Hubei Academy of Agricultural Sciences,Wuhan,430064)
出处
《分子植物育种》
CAS
北大核心
2024年第6期1842-1849,共8页
Molecular Plant Breeding
基金
国家现代农业产业技术体系建设专项(CARS-31-02A)
农业部华中作物有害生物综合治理重点实验室开放基金项目(2018ZTSJJ6)
湖北省中央引导地方科技发展专项(2019ZYYD034)共同资助。
