摘要
液态芯片技术以其多元同时检测的优势在生物分析领域得到了广泛的应用。然而,相比其他接近单分子检测灵敏度的生物分析检测技术,液态芯片技术的灵敏度还有待进一步提高。本研究以等离子磁性微球取代液态芯片技术中传统的微球,构建了具有荧光增强特性的新型液态芯片检测平台。研究表明,本文制备的等离子磁性荧光微球可以实现对特定荧光分子达到70倍的荧光增强效应,构建的新型液态芯片检测平台对蛋白类肿瘤标志物的检测灵敏度也可以提高2个数量级,为传统液态芯片技术检测灵敏度的提高提供了方法学借鉴。
As a signal amplification method,plasmon-enhanced fluorescence technology demonstrated significant potential in practical application in the field of environmental-and biological-assay.Therefore,it is imperative to develop a method for the scalable and reproducible synthesis plasmon-enhanced fluorescence carrier,which plays a vital role in the commercialization of the technology.In this study,plasmonic magnetic microbeads were prepared by magnetic loading and polydopamine coating and subsequent noble metal nano island functionalization using polymer microbeads as the template.It is found that the prepared microbeads can be rapidly separated from the solution under an external magnet,and more 70-fold fluorescence enhancement can be obtained for dye CF647.By comparing with polystyrene bead-based suspension microarray,100-fold detection sensitivity can be achieved for biomarker CEA protein.
作者
曹本妹
李鑫
余龙
袁超
CAO Benmei;LI Xin;YU Long;YUAN Chao(College of Environmental Science and Engineering,Guangdong University of Petrochemical Technology,Maoming 525000,China)
出处
《广东石油化工学院学报》
2024年第1期40-44,共5页
Journal of Guangdong University of Petrochemical Technology
基金
广东石油化工学院人才引进项目(2022rcyj2002)
茂名市科技计划项目(2023385)。
关键词
等离子荧光增强
微球
肿瘤标志物
液态芯片
plasmon-enhanced fluorescence
CEA
suspension microarray
microbead
