摘要
目的:分析乙型肝炎相关性肝癌患者T淋巴细胞、白介素-28B (IL-28B)及高尔基体蛋白73 (GP73)的表达及临床意义。方法:选取2018年10月—2021年10月商丘市第一人民医院收治的119例乙型肝炎相关性肝癌患者作为观察组,选取同期体检的112例健康人群作为对照组。比较两组研究对象血清IL-28B、GP73及T淋巴细胞水平,分析IL-28B、GP73、T淋巴细胞与病理特征及预后的关系。结果:观察组血清IL-28B、GP73及CD8^(+)表达水平均高于对照组,CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均低于对照组,差异有统计学意义(t=148.380、13.155、9.827、9.939、16.367、11.453,P<0.05)。不同年龄、性别、肿瘤直径的乙型肝炎病毒(HBV)相关性肝癌患者血清中IL-28B、GP73水平比较,差异无统计学意义(P>0.05)。有淋巴结转移、TNM分期Ⅲ~Ⅳ期、低分化、有血管浸润者血清中IL-28B、GP73水平高于无淋巴结转移、TNM分期Ⅰ~Ⅱ期、中/高分化、无血管浸润者,差异有统计学意义(P<0.05)。HBV相关性肝癌患者血清中CD3^(+)、CD4^(+)、CD8^(+)在不同年龄、性别、肿瘤直径中比较,差异无统计学意义(P>0.05),CD3^(+)、CD4^(+)、CD8^(+)水平在不同淋巴结转移情况、TNM分期、分化程度及血管浸润中比较,差异无统计学意义(P>0.05)。死亡者血清GP73、IL-28B水平均高于生存者,CD4^(+)/CD8^(+)指标低于生存者,差异有统计学意义(t=16.629、2.082、9.267,P<0.05)。结论:乙型肝炎相关性肝癌患者血清IL-28B、GP73及CD8^(+)表达水平明显升高,CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)指标下降,IL-28B、GP73及CD4^(+)/CD8^(+)水平与相关病理特征、预后具有密切的联系。
Objective:To analyze the expression and clinical significance of T lymphocytes,interleukin-28B(IL-28B)and Gol⁃gi protein 73(GP73)in patients with hepatitis B-associated hepatocellular carcinoma.Methods:119 cases of hepatitis B-associated hepatocellular carcinoma patients admitted to the hospital from October 2018 to October 2021 were selected to be included in the observation group,and 112 healthy people who underwent physical examination in the same period were selected to be included in the control group.Serum IL-28B,GP73 and T-lymphocyte levels were compared between the two groups,and the relationship between IL-28B,GP73 and T-lymphocytes and pathological features and prognosis was analyzed.Results:The serum IL-28B,GP73 and CD8^(+)expression levels of the observation group were higher than those of the control group,and CD3^(+),CD4^(+)and CD4^(+)/CD8^(+)were lower than those of the control group,and the differences were statistically significant(t=148.380,13.155,9.827,9.939,16.367,11.453;P<0.05).There was no statistically significant difference in the comparison of serum levels of IL-28B and GP73 in patients with HBV-associated hepatocellular carcinoma of different ages,genders and tumor diameters(P>0.05).Serum levels of IL-28B and GP73 were higher in those with lymph node metastasis,TNM stage III~IV,low differentiation,and with vascular infiltration than in those without lymph node metastasis,TNM stage I~II,moderate/high differentiation,and with or without vascular infiltration,and the difference was statistically significant(P<0.05).The differences in serum CD3^(+),CD4^(+),and CD8^(+)in patients with HBV-associated hepatocellular carcinoma were not statistically significant in different ages,genders,and tumor diameters(P>0.05).The differences in CD3^(+),CD4^(+),and CD8^(+)levels were not statistically significant in different lymph node metastasis,TNM stage,differentiation degree,and vascular infiltration(P>0.05).The serum GP73 and IL-28B levels of the deceased were higher than those of the survivors,and the CD4^(+)/CD8^(+)indexes were lower than those of the survivors,with statistically significant differences(t=16.629,2.082,9.267;P<0.05).Conclusion:Hepatitis B-associated hepatocellular carcinoma patients had significantly higher serum IL-28B,GP73 and CD8^(+)expression levels,and decreased CD3^(+),CD4^(+)and CD4^(+)/CD8^(+)indexes,and the levels of IL-28B,GP73 and CD4^(+)/CD8^(+)had a close association with the relevant pathologic features and prognosis.
作者
张馨心
刘亚清
崔永辉
许春进
Zhang Xinxin;Liu Yaqing;Cui Yonghui(Department of Gastroenterology,Shangqiu First People’s Hospital,Shangqiu,Henan,476000,China)
出处
《黑龙江医学》
2024年第6期675-678,共4页
Heilongjiang Medical Journal
