摘要
为了解广西北部湾海域织锦巴非蛤(Paphia textile)精巢发育、精子发生及成熟精子的超微结构,文章采用解剖观察、精巢组织切片、扫描和透射电镜技术进行了研究。结果表明,所采集织锦巴非蛤中未发现雌雄同体,观察的个体皆为雌雄异体,将其精巢发育划分为增殖期、生长期、成熟期、排放期、休止期5个时期,织锦巴非蛤精巢成熟与排放期在9月-翌年1月。精子发生分为精原细胞期、初级精母细胞期、次级精母细胞期、精细胞期、成熟精子期5个时期。织锦巴非蛤成熟精子由头部、中部和尾部组成,为典型的鞭毛型精子,全长约为46.04μm。精子头部分为顶体和精核,顶体为圆锥形,精核呈长圆柱形,前端直径约为0.71μm,后端直径约为1.34μm;中部由4个环绕成瓣状的线粒体与2个相互垂直的近、远端中心粒组成;尾部鞭毛长约38.8μm,直径约为0.21μm,外被质膜,为典型“9+2”双联体微管结构。研究结果为织锦巴非蛤的繁殖生物学、人工苗种繁育及种质资源保护提供研究基础。
In this study,anatomical observation,spermary tissue section,scanning and transmission electron microscopy techniques were used to study the spermary development,spermatogenesis and ultrastructure of mature sperm of Paphia textile in Beibu Gulf of Guangxi.The results showed that no hermaphrodites were found in the collected samples,and all the observed individuals were dioecious.The spermary development of P.textile was divided into five main stages:proliferating stage,growing stage,maturing stage,spawning stage,and resting stage.The maturing stage and spawning stage of P.textile was from September to January of the following year.Sperm development is divided into five stages:spermatogonia stage,primary spermatocyte stage,secondary spermatocyte stage,spermatid stage and mature sperm stage.The mature sperm of P.textile consisting of the head,middle and tail,overall length approximately 46.04μm.The sperm head is divided into the acrosome and the nucleus.The acrosome is conical in shape and the nucleus is long and cylindrical.The anterior end was 0.71μm in diameter and the posterior end was about 1.34μm in diameter.The middle part consisted of four mitochondria encircled in a circular shape and two mutually perpendicular proximal and distal centrioles.The tail flagellum was about 38.8μm long and 0.21μm in diameter,and the external plasma membrane was covered with a typical“9+2”duplex microtubule structure.The results of this study provide basic research information for the reproductive biology,artificial seed breeding and germplasm resource conservation of P.textile.
作者
徐炳杰
刘一鸣
邢清淦
连昌朋
吴韬
潘英
XU Bingjie;LIU Yiming;XING Qinggan;LIAN Changpeng;WU Tao;PAN Ying(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities,Nanning 530004,China)
出处
《热带海洋学报》
CAS
CSCD
北大核心
2024年第2期59-68,共10页
Journal of Tropical Oceanography
基金
广西创新驱动发展专项(桂科AA19254032)。
关键词
织锦巴非蛤
精巢发育
精子发生
超微结构
组织切片
Paphia textile
spermary development
spermatogenesis
ultrastructural
histological section
