摘要
Objective:Resveratrol polymer micelles with tissue adhesion were prepared and the content of resveratrol in the micelles was determined by HPLC.Method:The micelle adhesion experiment was carried out by polylysine orifice plate experiment and small animal fluorescence imaging method,and the micelle prescription was optimized to obtain resveratrol micelles with good adhesion.The separation was performed on a Shiseido SPOLAR C18 column(150 mm×4.6 mm,5μm)with methanol-water(42:58)as the mobile phase.The flow rate was 1.0 mL·min^(-1),the detection wavelength was 305 nm,the column temperature was 35℃,and the injection volume was 10μL.Results:Resveratrol micelles prepared with F127 alone had the best adhesion.The peak area and concentration of resveratrol had a good linear relationship in the concentration range of 10~200μg/mL(r=0.9996).The specificity,precision,recovery and stability all met the methodological requirements.Conclusion:In this experiment,resveratrol micelles with tissue adhesion were successfully prepared,and a method for the determination of resveratrol content in micelles was established.The method is accurate,rapid and simple.
基金
High Level Talent Program of Hainan Natural Science Foundation (821RC569)
National Natural Science Foundation-Regional Science Foundation Project (82060642)
Hainan Provincial Association of Science and Technology Youth Science and Technology Talent Innovation Program Project (QCXM202029)
Hainan Province Higher Education Science Research Project (Hnky2020-35)
National Natural Science Foundation-Youth Science Foundation Project (81502998)
Hainan Medical College 2022 College Student Innovation and Entrepreneurship Training Program Project (X202211810094)。
