摘要
目的:探讨无外周血CD34监测下聚乙二醇重组人粒细胞集落刺激因子(PEG-rhG-CSF)在血液肿瘤自体外周血干细胞动员中的采集时机及采集效果。方法:回顾性分析2017年8月至2022年1月福建医科大学附属第一医院收治的46例行自体外周血干细胞动员的血液恶性肿瘤患者。采用大剂量化疗联合PEG-rhG-CSF或重组人粒细胞集落刺激因子(G-CSF)动员方案,其中应用PEG-rhG-CSF动员的27例(PEG-rhG-CSF组),应用G-CSF动员的19例(G-CSF组),比较两组患者动员采集效果。结果:46例患者共采集86例次,PEG-rhG-CSF组与G-CSF组获得采集物的单个核细胞中位数分别为6.54(3.85-12.61)×10^(8)/kg和6.15(1.13-11.58)×10^(8)/kg(P>0.05),采集物CD34^(+)细胞数分别为11.44(1.33-65.02)×10^(6)/kg和4.95(0.30-24.02)×10^(6)/kg(P<0.05),采集时机分别为14(10-20)和14(4-22)d(P>0.05)。PEG-rhG-CSF组在外周血白细胞(WBC)≥10×10^(9)/L时单次所采集的产物CD34^(+)细胞数明显高于外周血WBC<10×10^(9)/L时采集的数量[19.04(2.85-65.02)×10^(6)/kg vs 6.22(0.81-34.86)×10^(6)/kg,(P<0.05)]。结论:采用PEG-rhG-CSF动员外周血干细胞单次采集足量CD34^(+)细胞成功率高,中位动员时间为14 d;在无外周血CD34监测情况下,外周血WBC≥10×10^(9)/L可以考虑作为单次采集足量干细胞的采集阈值。
Objective:To investigate the efficiency and optimal time of stem cell apheresis mobilized by pegylated recombinant human granulocyte colony stimulating factor(PEG-rhG-CSF)in autologous stem cell transplantation(ASCT)for hematological malignancies without monitoring pre-collection CD34^(+)cells.Methods:Forty-six patients underwent stem cell mobilization were retrospectively analyzed between August 2017 and January 2022 at the First Affiliated Hospital of Fujian Medical University.27 patients using high dose chemotherapy combined with PEG-rhG-CSF mobilization were enrolled in the PEG-rhG-CSF group,and other 19 patients mobilized with recombinant human granulocyte colony stimulating factor(G-CSF)were enrolled in G-CSF group.The mobilization and collection effects of the patients in two groups were compared.Results:A total of 46 patients underwent 86 apheresis procedures,the median amount of mononuclear cell(MNC)in the PEG-rhG-CSF group and G-CSF group was 6.54(3.85-12.61)×10^(8)/kg and 6.15(1.13-11.58)×10^(8)/kg,respectively(P>0.05),the total CD34^(+)cells of the grafts were 11.44(1.33-65.02)×10^(6)/kg and 4.95(0.30-24.02)×10^(6)/kg(P<0.05),with harvest timing of 14(10-20)days and 14(4-22)days,respectively(P>0.05).In the PEG-rhG-CSF group,there was a significant difference between the number of CD34^(+)cells collected when white blood cells(WBC)≥10×10^(9)/L and WBC<10×10^(9)/L,19.04(2.85-65.02)×10^(6)/kg and 6.22(0.81-34.86)×10^(6)/kg,respectively(P<0.05).Conclusion:Stem cells mobilization with PEG-rhG-CSF was highly efficient with a median mobilization time of 14 days.In the absence of peripheral blood CD34 monitoring,peripheral blood WBC≥10×10^(9)/L can be considered as a threshold for a single stem cell apheresis to collect sufficient stem cells.
作者
陈清娇
郑晓强
CHEN Qing-Jiao;ZHENG Xiao-Qiang(Department of Hematology,The First Affiliated Hospital,Fujian Medical University;Department of Hematology,National Regional Medical Center,Binhai Campus of the First Affiliated Hospital,Fujian Medical University,Fujian Key Laboratory of Laboratory Medicine,Fuzhou 350005,Fujian Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
2024年第2期556-560,共5页
Journal of Experimental Hematology
基金
福建省自然科学基金(2021J01710)。
关键词
自体外周血造血干细胞移植
造血干细胞动员
聚乙二醇重组人粒细胞集落刺激因子
autologous hematopoietic stem cell transplantation
stem cell mobilization
pegylated recombinant human granulocyte colony stimulating factor
