摘要
【目的】评价不同基因型培忠杉苗木的耐碱能力,研究其在碱胁迫环境中苗木生理生化指标的变化,为培忠杉苗木耐碱机理研究和苗木推广应用提供参考依据。【方法】以不同基因型(S1、S2、S3、S4和S5)培忠杉2.5年生扦插容器苗为试验材料,采用不同碱性盐溶液(pH值7、pH值8、pH值9和pH值10)浸泡容器育苗基质,测定分析各处理条件下苗木叶片的相对电导率、可溶性糖含量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、叶绿素含量及叶绿素a/b的动态变化过程。【结果】不同基因型、不同碱处理以及基因型与碱处理的交互作用对培忠杉苗木叶片相对电导率、可溶性糖含量、超氧化物酶(SOD)活性、丙二醛(MDA)含量、叶绿素含量的影响以及基因型和基因型与碱处理的交互作用对培忠杉苗木叶绿素a/b比值的影响均达到极显著水平(P <0.01)。随着碱处理时间的延长,S1、S3和S5基因型培忠杉苗木叶片相对电导率、可溶性糖含量、SOD活性、MDA含量、叶绿素含量及叶绿素a/b均呈先上升后下降的变化趋势。在碱处理57 d时,pH值10盐溶液处理条件下的5种基因型苗木叶片相对电导率、SOD活性、MDA含量均显著高于对照处理,但5种基因型苗木之间叶片叶绿素含量、叶绿素a/b值的差异并不显著。采用模糊数学隶属函数值法综合分析不同基因型苗木耐碱能力为S5> S4> S3> S1> S2。【结论】在碱胁迫处理条件下,不同基因型培忠杉苗木可以通过调节自身生理状态快速应对碱胁迫,但不同基因型培忠杉苗木的耐碱能力存在差异,表现在生理生化指标动态变化的差异。同时,用模糊数学隶属函数值法综合评价得出S5基因型苗木的耐碱能力最强。
【Objective】This research investigated the effects of alkali stress on physiology and biochemistry of different genotypes of×Taxodiomera peizhongii seedlings,to evaluate the alkali tolerance ability of different genotypes of seedlings,and provide reference basis for the study of alkali tolerance mechanism and the promotion and application of×T.peizhongii seedlings.【Method】The experiment used 2.5-age-old cuttage container seedlings of different genotypes of×T.peizhongii(S1,S2,S3,S4 and S5)as materials,which were treated with different concentrations alkaline salt solution(pH8,pH9 and pH10).The physiology indicators were measured to analyze the alkali tolerance ability among five genotypes of×T.peizhongii such as the content of relative conductivity,soluble sugar content,SOD activity,MDA content,chlorophyll content and chlorophyll a/b.The alkali tolerance of five genotypes was comprehensively evaluated by membership function method.【Result】The effects of different genotypes,different alkali treatments,and the interaction of genotypes and alkali on the relative electrical conductivity,soluble sugar content,SOD activity,MDA content,chlorophyll content,genotype and the interaction between genotype and alkali on the chlorophyll a/b ratio×T.peizhongii reached an extremely significant difference(P<0.01).With the extension of treatment time,the relative conductivity,soluble sugar content,SOD activity,MDA content,chlorophyll content and chlorophyll a/b of S1,S3 and S5 genotypes of×T.peizhongii seedlings increased first and then decreased under alkali treatment.After 57 days of treatment,the relative conductivity,SOD activity and MDA content of the leaves of the five genotypes of×T.peizhongii under pH10 treatment were significantly higher than those under CK treatment,but the chlorophyll content and chlorophyll a/b value of the leaves of the five genotypes were not significantly different.Integrated analysis of fuzzy membership function values showed that the relationship of alkali resistance of five genotype was:S5>S4>S3>S1>S2.【Conclusion】Different genotypes seedlings of×T.peizhongii regulated their physiology to cope with alkali stress quickly under different alkali stress treatment.However,there were differences in the alkaline resistance of different genotypes of×T.peizhongii seedlings,which were reflected in the differences in dynamic changes of physiological and biochemical indicators.Meanwhile,the comprehensive evaluation of the physiological index test results showed that the S5 genotype seedlings had the strongest alkali resistance.
作者
邱雨后
陈金玲
谢敏譞
史锋厚
张于卉
孙燕
尹中明
沈永宝
QIU Yuhou;CHEN Jinling;XIE Minxuan;SHI Fenghou;ZHANG Yuhui;SUN Yan;YIN Zhongming;SHEN Yongbao(College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Shanghai Forestry Station,Jing’an 200072,Shanghai,China;Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,Jiangsu,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2024年第1期97-108,共12页
Journal of Central South University of Forestry & Technology
基金
江苏省林业科技创新与推广项目(LYKJ[2022]21)
上海市绿化和市容管理局科技攻关项目(G191207)
江苏高校优势学科建设工程资助项目(PAPD)。
关键词
培忠杉
基因型
碱胁迫
生理生化
×Taxodiomera peizhongii
genotype
alkaline stress
physiological and biochemical
