摘要
目的 对PVG大鼠进行生化标记基因的测定。方法 依据国家标准GB/T 14927.1—2008《实验动物近交系小鼠、大鼠生化标记检测法》测定,随机抽取4只PVG大鼠,取(肺、肾、小肠、睾丸)组织器官进行样品制备,用醋酸纤维薄膜电泳法检测11个生化位点(Hbb、Cs1、Alp1、Akp1、Esl、Es3、Es4、Es6、Es8、Es9和Esl0)。结果 PVG大鼠11个位点的基因型分别是Hbb为b型,Cs1为b型,Alp1为b型,Akp1为a型,Esl为a型,Es3为d型,Es4为b型,Es6为b型,Es8为b型,Es9为a型和Esl0为a型,且均为纯合。结论 PVG大鼠遗传生化标记结果符合近交系特征。
Objective Biochemical marker genes were determined in PVG rats.Method According to the national standard GB/T 14927.1—2008“Biochemical Marker Detection Method for Inbred Mice and Rats in Laboratory Animals”,4 PVG rats were randomly selected to collect tissues and organs(lung,kidney,small intestine,testis)for sample preparation.Eleven biochemical sites(Hbb,Cs1,Alp1,Akp1,Esl,Es3,Es4,Es6,Es8,Es9 and Esl0)were detected by acetate fiber film electrophoresis.Result The genotypes of 11 PVG rat loci were Hbb type b,Cs1 type b,Alp1 type b,Akp1 type a,Esl type a,Es3 type d,Es4 type b,Es6 type b,Es8 type b,Es9 type a and Esl0 type a,all of which were homozygous.Conclusion The result of genetic and biochemical markers in PVG rats were consistent with the characteristics of inbred lines.
作者
朱婉月
王洪
刘巍
李欢
赵蓝
董浩
冯宾
岳秉飞
魏杰
左琴
ZHU Wanyue;WANG Hong;LIU Wei;LI Huan;ZHAO Lan;DONG Hao;FENG Bin;YUE Bingfei;WEI Jie;ZUO Qin(National Institutes for Food and Drug Control,Department of Laboratory Animal Quality Testing,National Rodent Laboratory Animal Resources Center,Beijing 102629,China)
出处
《实验动物科学》
2024年第1期29-32,共4页
Laboratory Animal Science
基金
国家重点研发计划(2021YFF0703200)。
