摘要
目前甲真菌病被认为是全球分布的主要公共卫生问题之一。甲真菌病的诊断依靠典型临床表现、真菌学检查(真菌镜检和真菌培养)以及甲病理检查。传统的真菌学检测方法在精准快速地鉴定真菌种类方面存在较大困难。免疫学诊断、基于PCR的分子鉴定、基因芯片技术、LAMP、MALDI-TOF MS及光谱学鉴定等新兴诊断技术具有快速、简便、精准的优点,能够克服传统诊断技术的局限性。本文阐述甲真菌病真菌学检测方法的研究进展,旨在发现和总结传统诊断方法与新兴诊断方法的优缺点,以便新兴检测方法的研究与发展。
Presently,onychomycosis is one of the major global public health issues.The diagnosis of onychomycosis relies on typical clinical signs,mycological examination(fungal microscopy,culture)and pathological analysis.The traditional methods are difficult to precisely and rapidly identify fungal species.Emerging technologies,such as PCR-based molecular identification,gene chip,LAMP,MALDI-TOF MS and spectroscopy,exhibit advantages of rapidity,simplicity and accuracy,and overcome the limitations of traditional methods.This paper summarizes the advantages and disadvantages of traditional and modern methods for mycological detection in order to facilitate the development of new detection methods.
作者
曹冰莹
廖德君
杨茜
杨政敏
CAO Bingying;LIAO Dejun;YANG Xi;YANG Zhengmin(Qiannan Medical College for Nationnalities,2.Key Laboratory of Human Parasitic Diseases in Qiannan Prefecture,Duyun 558000,China)
出处
《皮肤性病诊疗学杂志》
2024年第2期129-135,共7页
Journal of Diagnosis and Therapy on Dermato-venereology
基金
贵州省卫生健康委科学技术基金项目(gzwkj2023-578)
黔南州科技计划项目(黔南科合[2021]26号)
黔南民族医学高等专科学校科研基金资助项目(Qnyz202109)。
