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褪黑素对高温胁迫玉米叶片光合特性与抗氧化酶活性的调控

Regulation of Melatonin on Photosynthetic Characteristics and Antioxidant Enzyme Activities in Maize Leaves under High Temperature Stress Condition
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摘要 2022年以安科985为试验材料,在玉米灌浆期自然高温胁迫条件下(2022年夏高温持续时间长、范围广、强度大、极端性强),灌施不同浓度褪黑素(MT)溶液,0(CK)、100(MT100)、200(MT200)、300(MT300)μmol/L,研究不同处理下玉米叶片光合特性、荧光特性、抗氧化酶活性与产量形成的影响。结果表明,褪黑素处理显著增加了玉米行粒数、穗粗、穗长、百粒重和单株产量,降低了秃尖长度。褪黑素处理后叶片SPAD值、N含量、净光合速率、气孔导度和蒸腾速率显著增加,胞间二氧化碳浓度显著降低。与对照相比,褪黑素处理下ΦPSⅡ、ETR、qN、qP和F_(v)/F_(m)均显著上升,Y(N0)和F_(0)显著下降。200μmol/L处理显著提高叶片抗氧化酶活性(SOD、POD、CAT),丙二醛MDA含量显著降低。褪黑素处理后,干物质积累量和干物质向子粒的分配比例显著增加。自然高温胁迫下,褪黑素处理可增强玉米抗性,以200μmol/L效果最佳。 In 2022,Anke 985 was used as the experimental material.Under the condition of natural high tem⁃perature stress at the filling stage of maize(long duration,wide range,high intensity and strong extremeness of high temperature in summer of 2022),different concentrations of melatonin(MT)solution 0(CK),100(MT100),200(MT200),300(MT300)μmol/L were set to study the effects of different treatments on photosynthetic characteristics,fluorescence characteristics,antioxidant enzyme activities and yield formation of maize leaves.The results showed that Melatonin treatment significantly increased the number of kernels per row,ear diameter,ear length,100-grain weight and yield per plant,and decreased the length of bald tip.Ccompared with the control.The SPAD value,N content,net photosynthetic rate,stomatal conductance and transpiration rate of leaves increased significantly after melatonin treatment,and the intercellular carbon dioxide concentration decreased significantly.Compared with the control,ΦPSII,ETR,qN,qP and F_(v)/F_(m) were significantly increased under melatonin treatment.Compared with the control,and Y(N0)and F_(0) decreased significantly.The antioxidant enzyme activities(SOD,POD,CAT)of leaves were significantly increased by 200μmol/L treatment,and the MDA content was significantly decreased.After mela⁃tonin treatment,dry matter accumulation and dry matter distribution to grains increased significantly.Under natural high temperature stress,melatonin treatment can enhance maize resistance,and 200μmol/L is the best.
作者 余梦奇 路梦莉 张雅婷 程嘉慧 李文阳 YU Meng-qi;LU Meng-li;ZHANG Ya-ting;CHENG Jia-hui;LI Wen-yang(College of Agronomy,Anhui Science and Technology University,Fengyang 233100,China)
出处 《玉米科学》 CAS CSCD 北大核心 2024年第1期90-99,共10页 Journal of Maize Sciences
基金 安徽高校自然科学研究重大项目(KJ2020ZD010) 安徽省财政农业科技成果转化应用项目(2021ZH015)。
关键词 玉米 褪黑素 自然高温 光合荧光特性 抗氧化酶 产量 Maize Melatonin Natural high temperature Photosynthetic fluorescence characteristic Antioxi⁃dant enzyme Yield
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