摘要
【目的】探讨HY-041菌株的除草潜力及生物学特性,为其用于工业化生产提供理论依据。【方法】通过离体接种法,测定了菌株HY-041对野燕麦(Avena fatua L.)、藜(Chenopodium album L.)、密花香薷(Elsholtzia densa Benth.)、猪秧秧(Galium aparine L.)、冬葵(Malva verticillata L.var.crispa)、酸模叶蓼(Polygonum lapathifolium L.)和反枝苋(Amaranthus retroflexus L.)7种杂草叶片的致病力。采用生长速率法和孢子计数法,考察不同培养基、光照、温度、碳源、氮源、pH及致死温度等因子对菌丝生长和产孢量的影响。【结果】HY-041菌株接种离体叶片7 d致病性表现为:酸模叶蓼>冬葵>野燕麦>藜>猪秧秧>反枝苋>密花香薷。HY-041菌株在PSA培养基中生长发育最快,且菌丝稠密,菌株最适生长温度为20℃,最适产孢温度为25℃,在持续黑暗环境下,更有利于菌丝生长和孢子的产生,最适碳源为肌醇,最适氮源为硝酸钾和硝酸钠,菌株适宜生长pH值为9,最适产孢pH为8,致死温度为55℃下水溶10 min。【结论】本试验明确了HY-041菌株在离体条件下对7种杂草的致病性及该菌的生物学特性,通过优化提高了菌株的产孢量,为生防制剂的研究奠定理论基础。
【Objective】To study the herbicidal potential and biological characteristics of HY-041 strain and provide theoretical basis for its industrial production.【Method】The pathogenicity of strain HY-041 to seven weeds(Avena fatua L.,Chenopodium album L.,Elsholtzia densa Benth.,Galium aparine L.,Malva verticillate L.var.crispa,Polygonum lapathifolium L.and Amaranthus retroflexus L.)was determined by in vitro inoculation.The effects of different media,light,temperature,carbon source,nitrogen source,pH and lethal temperature on mycelial growth and spore production were studied by growth rate method and spore counting method.【Result】The pathogenicity of strain HY-041 inoculated on isolated leaves for 7 days was as follows:P.lapathifolium>M.verticillata>A.fatua>C.album>G.aparine>A.retroflexus>E.densa.The HY-041 strain grew fastest and had dense mycelia in PSA medium,the optimum growth temperature of the strain is 20℃,the optimum sporulation temperature is 25℃,in a continuous dark environment,more conducive to mycelial growth and spore production,the optimum carbon source is inositol,the optimum nitrogen source is potassium nitrate and sodium nitrate,the suitable growth pH of the strain is 9,the optimum sporulation pH is 8,the mycelial lethal temperature is 55℃/10 min.【Conclusion】The pathogenicity of HY-041 strain on 7 species of weeds in vitro and the biological characteristics of the strain were clarified,and the spore production of strain HY-041 was increased by optimization,which provided a theoretical basis for the study of biocontrol agents.
作者
李欢
朱海霞
LI Huan;ZHU Haixia(Academy of Agriculture and Forestry Sciences,Qinghai University Xining Crop Pest Scientific Observation and Experimental Station,Ministry of Agriculture,Key Laboratory of Comprehensive Control of Agricultural Pests,Xining 810016,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2024年第1期194-202,210,共10页
Journal of Gansu Agricultural University
基金
青海省科技厅国际合作专项(2023-HZ-808)。
关键词
除草潜力
生物学特性
产孢量
生防制剂
herbicidal potential
biological properties
spore production
biocontrol preparations
