摘要
【目的】探究外源褪黑素(Melatonin,MT)对干旱胁迫下园林小菊的缓解作用及适宜浓度,为干旱和半干旱地区种植园林小菊提供理论支持和参考价值。【方法】以园林小菊绚秋凝红为试材,使用20%PEG作为干旱胁迫渗透剂,通过叶面喷施不同浓度MT(50、100、150、200μmol/L),研究MT对干旱胁迫下园林小菊幼苗生长及生理指标的影响。【结果】干旱胁迫条件下,外源喷施100μmol/L MT可有效缓解干旱胁迫对园林小菊幼苗造成的伤害。园林小菊幼苗叶绿素含量、叶片相对含水量较PEG处理分别增加37.4%、69.1%,相对电导率(REC)和丙二醛含量(MDA)分别降低55.7%和42.8%,过氧化氢(H_(2)O_(2))增加75.0%,脯氨酸(Pro)增加60.6%,超氧化物歧化酶(SOD)、过氧化物歧化酶(POD)、过氧化氢酶(CAT)活性分别是PEG处理的1.9倍、2倍、3.2倍。通过主成分分析结果可知,外源MT对园林小菊叶绿素含量、叶片相对含水量和地上部鲜质量产生较大影响。【结论】外源喷施100μmol/L MT能够有效缓解干旱胁迫对园林小菊绚秋凝红所造成的伤害,提高扦插幼苗的耐旱性。
【Objective】The study aimed at exploring the alleviation effect of exogenous melatonin(MT)on garden chrysanthemum under drought stress and screening its suitable concentration,providing reference for planting garden chrysanthemum in arid and semi-arid areas.【Method】With the garden chrysanthemum Xuanqiu ninghong as the test material and the 20%PEG as a drought stress penetrant,the different concentrations of MT(50,100,150,200μmol/L)were sprayed on the leaves to study the its effects on the growth and physiological indexes of chrysanthemum seedlings under drought stress.【Result】Under the drought stress conditions,the exogenously spraying of 100μmol/L MT could effectively alleviate the damage caused by drought stress to the garden chrysanthemum seedlings.Compared with the PEG treatment,the chlorophyll content and relative water content of the leaves increased by 37.4%and 69.1%,respectively,and the relative conductivity(REC)and malonaldehyde content(MDA)decreased by 55.7%and 42.8%,respectively.Hydrogen peroxide(H_(2)O_(2))increased by 75.0%,and proline(Pro)by 60.6%.The activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)were 1.9,2,and 3.2 times of PEG treatment.The results of the Principal Component Analysis showed that exogenous MT mainly had a great influence on the chlorophyll content and relative water content of leaves,and on the fresh weight of shoots of garden chrysanthemum.【Conclusion】This study showed that the exogenously spraying of 100μmol/L MT could effectively alleviate the damage to the garden chrysanthemum Xuanqiu ninghong caused by drought stress,improving the drought tolerance of the cutting seedlings.
作者
罗艳
虎淘淘
高晓芬
赵钰磊
张黎
严瑞
LUO Yan;HU Taotao;GAO Xiaofen;ZHAO Yulei;ZHANG Li;YAN Rui(College of Enology and Horticulture,Ningxia University,Yinchuan 750021,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2024年第1期211-218,共8页
Journal of Gansu Agricultural University
基金
宁夏回族自治区重点研发计划项目“彩菊切花种苗快繁及精细化栽培技术集成示范”(2022BBF03035)。
关键词
园林小菊
外源褪黑素
PEG干旱胁迫
生理指标
garden chrysanthemum
exogenous melatonin
PEG drought stress
physiological index
