摘要
分支酸合成酶AroC是莽草酸途径中芳香族化合物合成的关键酶,探明短短芽胞杆菌aroC基因的特征及转录调控因子可为短短芽胞杆菌作用机理研究奠定基础。从短短芽胞杆菌FJAT-0809-GLX中克隆了aroC基因,进行生物信息学分析和转录因子预测。短短芽胞杆菌FJAT-0809-GLX中aroC基因序列长度为1 164 bp, GenBank登录号为OR475562。短短芽胞杆菌FJAT-0809-GLX与Brevibacillus brevis NBRC 100599的aroC基因序列同源性最高,为98.88%,与Brevibacillus brevis HNCS-1的AroC氨基酸序列同源性最高,为100%;其编码的蛋白为亲水性蛋白,无信号肽,定位于细胞质。靶向BbaroC的转录因子共有23个,分别来自10个物种,Escherichia coli(Strain K12 MG1655)和Bacillus subtilis(Strain 168)分别预测到12个和3个转录因子。BbaroC在短短芽胞杆菌抑菌活性物质产生中可能发挥着重要作用,这为其在农业上的广泛应用提供依据。
The chorismic acid synthetase AroC is the key enzyme for the synthesis of aromatic compounds in the shikimic acid pathway.Identifying the characteristics of aroC gene in Brevibacillus brevis and its transcriptional regulators will lay a foundation for the study of the mechanism of action of Brevibacillus brevis.The aroC gene was cloned from Brevibacillus brevis FJAT-0809-GLX,and then the bioinformatics analysis and the prediction of transcription factor were carried out.The sequence length of aroC gene in Brevibacillus brevis FJAT-0809-GLX was 1164 bp,and the GenBank accession number was OR475562.The aroC gene sequence of Brevibacillus brevis FJAT-0809-GLX had the highest homology with that of Brevibacillus brevis NBRC 100599,which was 98.88%.The amino acid sequence of AroC had the highest homology with Brevibacillus brevis HNCS-1,which was 100%.Its encoded protein was a hydrophilic protein without signal peptide,which was located in the cytoplasm.A total of 23 transcription factors targeting BbaroC were identified from 10 species,with 12 and 3 transcription factors predicted from Escherichia coli(Strain K12 MG1655)and Bacillus subtilis(Strain 168),respectively.BbaroC may play an important role in the production of antibacterial active substances of Brevibacillus brevis,which would provide basis for its wide application in agriculture.
作者
车建美
赖恭梯
许恒
贺丽媛
李思雨
陈冰星
王阶平
刘波
赖呈纯
CHE Jian-mei;LAI Gong-ti;XU Heng;HE Li-yuan;LI Si-yu;CHEN Bing-xing;WANG Jie-ping;LIU Bo;LAI Cheng-chun(Institute of Resources,Environment and Soil Fertilizer,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350002,China;Institute of Agricultural Products Processing,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350002,China)
出处
《福建农业科技》
CAS
2024年第1期8-17,共10页
Fujian Agricultural Science and Technology
基金
福建省科技计划省属公益类项目(2021R1034001)
福建省人民政府、中国农业科学院“5511”协同创新工程(XTCXGC2021019)。
关键词
短短芽胞杆菌
分支酸合成酶
氨基酸
莽草酸途径
转录因子
Brevibacillus brevis
Chorismic acid synthetase
Amino acid
Shikimic acid pathway
Transcription factor
