摘要
目的探讨乳腺癌中四跨膜蛋白8(Tspan8)、整合素α5(ITGA5)的表达与临床病理特征及预后的关系。方法将2018年4月至2020年4月该院收治的136例乳腺癌患者纳入研究。收集患者乳腺癌组织标本及对应的癌旁组织标本。采用免疫组化法检测Tspan8、ITGA5在乳腺癌组织及对应癌旁组织中的表达情况,分析Tspan8、ITGA5表达情况与乳腺癌患者临床病理特征的关系。对出院的乳腺癌患者行连续36个月的随访,记录患者生存状况,比较不同临床病理特征乳腺癌患者的3年生存率。采用多因素Cox回归分析乳腺癌患者腋窝淋巴结转移的影响因素。结果乳腺癌组织的Tspan8、ITGA5阳性表达率高于癌旁组织(P<0.05)。低分化、肿瘤最大径≥3 cm、TNM分期为Ⅲ~Ⅳ期、有腋窝淋巴结转移、其他分子分型的乳腺癌患者Tspan8、ITGA5阳性表达率高于中/高分化、肿瘤最大径<3 cm、TNM分期为Ⅰ~Ⅱ期、无腋窝淋巴结转移、三阴性分型的乳腺癌患者(P<0.05)。Tspan8、ITGA5阳性表达患者的3年生存率分别低于Tspan8、ITGA5阴性表达患者,差异均有统计学意义(P<0.05)。低分化、TNM分期为Ⅲ~Ⅳ期、有腋窝淋巴结转移、其他分子分型及Tspan8、ITGA5阳性表达患者的3年生存率低于中/高分化、TNM分期为Ⅰ~Ⅱ期、无腋窝淋巴结转移、三阴性分型及Tspan8、ITGA5阴性表达患者(P<0.05)。TNM分期为Ⅲ~Ⅳ期(HR=2.289,95%CI:1.519~3.447)、其他分子分型(HR=2.622,95%CI:1.744~3.942)及Tspan8阳性表达(HR=3.622,95%CI:2.159~6.077)、ITGA5阳性表达(HR=3.142,95%CI:2.022~4.884)均为乳腺癌患者腋窝淋巴结转移的危险因素(P<0.05)。结论Tspan8、ITGA5在乳腺癌患者中呈高表达,均与患者临床病理特征及预后有关。
Objective To investigate the expressions of transmembrane protein 8(Tspan8)and integrinα5(ITGA5)in breast cancer and their relationship with clinicopathological features and prognosis.Methods A total of 136 breast cancer patients admitted to the hospital from April 2018 to April 2020 were enrolled in the study.Breast cancer tissue samples and corresponding paracancerous tissue samples were collected.The expressions of Tspan8 and ITGA5 in breast cancer tissues and corresponding adjacent tissues were detected by immunohistochemistry.The relationship between the expressions of Tspan8 and ITGA5 and the clinicopathological characteristics of breast cancer patients was analyzed.The discharged breast cancer patients were followed up for 36 months,and the survival status of the patients was recorded.The 3-year survival rate of breast cancer patients with different clinicopathological characteristics was compared.Multivariate Cox regression was used to analyze the influencing factors of axillary lymph node metastasis in breast cancer patients.Results The positive expression rates of Tspan8 and ITGA5 in breast cancer tissues were higher than those in adjacent tissues(P<0.05).The positive expression rates of Tspan8 and ITGA5 in breast cancer patients with poor differentiation,tumor maximum diameter≥3 cm,TNM stageⅢ-Ⅳ,axillary lymph node metastasis and other molecular subtypes were higher than those in moderate/well differentiation,tumor maximum diameter<3 cm,TNM stageⅠ-Ⅱ,no axillary lymph node metastasis and triple negative subtype(P<0.05).The 3-year survival rate of patients with positive expression of Tspan8 and ITGA5 was significantly lower than that of patients with negative expression of Tspan8 and ITGA5(P<0.05).The 3-year survival rate of patients with poor differentiation,TNM stageⅢ-Ⅳ,axillary lymph node metastasis,other molecular subtypes and positive expressions of Tspan8 and ITGA5 were lower than those of patients with moderate/high differentiation,TNM stageⅠ-Ⅱ,no axillary lymph node metastasis,triple negative subtype and negative expressions of Tspan8 and ITGA5(P<0.05).TNM stageⅢ-Ⅳ(HR=2.289,95%CI:1.519-3.447),other molecular subtypes(HR=2.622,95%CI:1.744-3.942),Tspan8 positive expression(HR=3.622,95%CI:2.159-6.077)and ITGA5 positive expression(HR=3.142,95%CI:2.022-4.884)were risk factors for axillary lymph node metastasis in breast cancer patients(P<0.05).Conclusion Tspan8 and ITGA5 are highly expressed in breast cancer patients,which are related to the clinicopathological characteristics and prognosis of patients.
作者
王秦西
车康明
郭千弘
WANG Qinxi;CHE Kangming;GUO Qianhong(Department of Cancer Surgery,Tianshui First People′s Hospital,Tianshui,Gansu 741000,China)
出处
《国际检验医学杂志》
CAS
2024年第6期711-715,721,共6页
International Journal of Laboratory Medicine
基金
天水市科技计划项目(2022-SHFZKJK-3252)。
关键词
乳腺癌
四跨膜蛋白8
整合素Α5
腋窝淋巴结转移
breast cancer
tetratransmembrane protein 8
integrinα5
axillary lymph node metastasis
