摘要
目的探讨糖尿病肾病患者血清长链非编码RNA(lncRNA)核丰富转录本1(NEAT1)、微小RNA miR-23c水平与糖尿病肾病(DN)病情进展的关系。方法将该院2019年5月至2020年5月收治的136例DN患者纳入研究作为DN组。另选取58例同期于该院进行体检的健康者作为对照组。采用实时荧光定量PCR(qPCR)检测两组受试者血清lncRNA NEAT1、miR-23c、肾损伤分子-1(KIM-1)、中性粒细胞明胶酶相关载脂蛋白(NGAL)、肿瘤坏死因子-α(TNF-α)mRNA、白细胞介素-6(IL-6)mRNA水平。采用Pearson/Spearman相关分析DN患者血清lncRNA NEAT1、miR-23c与KIM-1、NGAL、TNF-α、IL-6 mRNA水平及eGFR的相关性。对DN患者进行CKD分期,比较不同CKD分期患者血清lncRNA NEAT1、miR-23c和KIM-1、NGAL、TNF-α、IL-6 mRNA水平,采用多元有序Logistic回归分析血清lncRNA NEAT1、miR-23c水平是否为DN病情进展的影响因素。结果与对照组比较,DN组血清lncRNA NEAT1和KIM-1、NGAL、TNF-α、IL-6 mRNA水平升高,miR-23c、估算的肾小球滤过率(eGFR)降低,差异均有统计学意义(P<0.05)。G1~G5期DN患者血清lncRNA NEAT1和KIM-1、NGAL、TNF-α、IL-6 mRNA水平均依次升高,miR-23c水平依次降低(P<0.05)。DN患者血清lncRNA NEAT1与KIM-1、NGAL、TNF-α、IL-6 mRNA水平呈正相关(P<0.05),与miR-23c、eGFR呈负相关(P<0.05);血清miR-23c水平与KIM-1、NGAL、TNF-α、IL-6 mRNA水平呈负相关(P<0.05),与eGFR呈正相关(P<0.05)。lncRNA NEAT1(OR=2.177,95%CI:2.113~2.441)为DN病情进展的独立危险因素,miR-23c(OR=0.595,95%CI:0.543~0.726)为独立保护因素(P<0.05)。结论DN患者血清lncRNA NEAT1水平的升高及miR-23c水平的降低均与DN病情进展密切相关。
Objective To investigate the relationship between serum levels of long non-coding RNA(lncRNA)nuclear-enriched abundant transcript 1(NEAT1)and microRNA miR-23c in patients with diabetic nephropathy(DN).Methods A total of 136 DN patients admitted to the hospital from May 2019 to May 2020 were enrolled in the study as the DN group.Fifty-eight healthy people who underwent physical examination in the hospital during the same period were enrolled as the control group.Real-time fluorescence quantitative PCR(qPCR)was used to detect serum lncRNA NEAT1,miR-23c,kidney injury molecule-1(KIM-1),neutrophil gelatinase-associated lipocalin(NGAL),tumor necrosis factor-α(TNF-α)mRNA and interleukin-6(IL-6)mRNA in the two groups.Pearson/Spearman correlation was used to analyze the correlation of serum lncRNA NEAT1 and miR-23c with KIM-1,NGAL,TNF-α,IL-6 mRNA levels and eGFR in DN patients.DN patients were divided into different CKD stages,and the levels of serum lncRNA NEAT1,miR-23c,KIM-1,NGAL,TNF-α,and IL-6 mRNA in patients in different CKD stages were compared.Multivariate ordered Logistic regression was used to analyze whether serum levels of lncRNA NEAT1 and miR-23c were influencing factors for the progression of DN.Results Compared with the control group,the serum levels of lncRNA NEAT1,KIM-1,NGAL,TNF-αand IL-6 mRNA in the DN group were increased,while miR-23c and estimated glomerular filtration rate(eGFR)were decreased,and the differences were all statistically significant(P<0.05).The serum levels of lncRNA NEAT1,KIM-1,NGAL,TNF-αand IL-6 mRNA in DN patients in G1-G5 stages were increased in order,and the level of miR-23c was decreased in order(P<0.05).Serum lncRNA NEAT1 in DN patients was positively correlated with KIM-1,NGAL,TNF-αand IL-6 mRNA levels(P<0.05),and negatively correlated with miR-23c and eGFR(P<0.05).The level of serum miR-23c was negatively correlated with the mRNA levels of KIM-1,NGAL,TNF-αand IL-6(P<0.05),and positively correlated with eGFR(P<0.05).lncRNA NEAT1(OR=2.177,95%CI:2.113-2.441)was an independent risk factor for DN progression,while miR-23c(OR=0.595,95%CI:0.543-0.726)was an independent protective factor(P<0.05).Conclusion Elevated serum lncRNA NEAT1 levels and reduced miR-23c levels in DN patients are closely associated with the progression of DN disease.
作者
吴多培
杨佳玲
陈文玉
贾莉
WU Duopei;YANG Jialing;CHEN Wenyu;JIA Li(Department of Hemodialysis,People′s Hospital of Lingshui Li Autonomous County,Linshui,Hainan 572400,China;Department of Nephrology,People′s Hospital of Lingshui Li Autonomous County,Linshui,Hainan 572400,China)
出处
《国际检验医学杂志》
CAS
2024年第6期727-732,共6页
International Journal of Laboratory Medicine
