摘要
【目的】通过根际可培养细菌多样性研究马铃薯对马铃薯块茎蛾(Phthorimaea operculella)取食胁迫的生理响应,为植物—蛀食性害虫—根际微生物互作效应及马铃薯块茎蛾的防治提供科学依据。【方法】室内盆栽青薯9号马铃薯,接种马铃薯块茎蛾3龄幼虫,分别于取食胁迫3、7和14 d时对马铃薯根际土壤进行取样,采用LB培养基分离培养根际土壤细菌,结合细菌形态特征及16S rRNA测序法鉴定细菌种类,分析可培养细菌的组成结构及多样性。【结果】马铃薯块茎蛾取食胁迫改变了马铃薯根际土壤细菌群落的组成结构及多样性,其中,胁迫3 d时马铃薯根际土壤细菌较对照多3种[海水罗塞略莫拉氏菌(Rossellomorea aquimaris)、长孢黄色链霉菌(Streptomyces longisporoflavus)和阿尔及利亚微杆菌(Microbacterium algeriense)];胁迫7 d时,较对照多2种[稻壳罗塞略莫拉氏菌(R.oryzaecorticis)和马氏副球菌(Paracoccus marcusii)];胁迫14 d时,较对照多2种[产吲哚金黄杆菌(Chryseobacterium indologenes)和布鲁氏菌根瘤菌(Brucella rhizospha)]。马铃薯块茎蛾取食胁迫后马铃薯根际可培养细菌的相对多度发生变化,其中在胁迫第3天变化程度达到最大。在取食胁迫3、7和14 d时,放线菌门(Actinobacteria)细菌相对多度分别增加23.49%、12.56%和28.06%,节杆菌属(Arthrobacter)相对多度分别增加103.50%、65.37%和28.57%。【结论】马铃薯块茎蛾取食胁迫可影响马铃薯根际细菌群落的物种组成结构及多样性,其中节杆菌属细菌的相对多度明显增加。研究结果为马铃薯根际对害虫取食胁迫的响应及马铃薯根际功能细菌的筛选提供基础。
[Purpose]The physiological response of potato to the feeding stress of Phthorimaea operculella was studied through the diversity of rhizosphere culturable bacteria,so as to provide sc-ientific basis for the interaction of plant-boring insect-rhizosphere microorganism and the management of P.operculella.[Methods]Indoor potted potato“Qingshu No.9”was inoculated with the 3rd instar P.operculella larvae of potato tuber moth.Soil samples were taken from the rhizosphere of potato under feeding stress for 3,7 and 14 days,and soil bacteria were isolated and cultured on LB medium.Morphological characteristics and 16S rRNA sequencing were used to identify the species of bacteria,and the composition and diversity of culturable bacteria were analyzed.[Results]P.operculella feeding stress changed the composition and diversity of rhizosphere soil bacterial.For 3 daysfeeding stress,three species of bacteria were specific compared with the control(Rossellomorea aquimaris,Streptomyces longisporoflavus,and Microbacterium algeriense);for 7 days-feeding stress,there were two species more than that of control(R.oryzaecorticis and Paracoccus marcusii);and for 14 days-feeding stress,there were two more species(Chryseobacterium indologenes and Brucella rhizospha).The relative abundance of rhizosphere soil culturable bacteria of potato changed after the feeding of P.operculella,and the greatest change was at 3 days-feeding stress.On the 3,7,14 daysfeeding stress,the relative abundance of Actinomycete bacteria increased by 23.49%,12.56%and 28.06%,respectively;and the relative abundance of Arthrobacter bacteria increased by 103.50%,65.37%and 28.57%,respectively.[Conclusion]The feeding stress of P.operculella affects the species and diversity of the rhizosphere bacteria community of potato,and the relative abundance of bacteria in Arthrobacter increases obviously.The results provide a basis for the response of potato rhizosphere to pest feeding stress and the screening of functional bacteria in potato rhizosphere.
作者
李俊逸
矣晓翠
刘福翠
姚遥
祝春月
刘一依
尹丽肖
肖关丽
LI Junyi;YI Xiaocui;LIU Fucui;YAO Yao;ZHU Chunyue;LIU Yiyi;YIN Lixiao;XIAO Guanli(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第1期44-53,共10页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(32360711,31760519)
国家重点研发计划课题(2021YFD1400705)。
关键词
马铃薯
马铃薯块茎蛾
生物胁迫
根际细菌
potato
Phthorimaea operculella
biological stress
rhizobacteria
