摘要
目的 检测血小板(PLT)抗体在妊娠期妇女血清中的表达情况,探讨分析该人群PLT抗体阳性的相关影响因素。方法 选取2 790例妊娠期妇女为研究对象,采用红细胞固相凝集法进行PLT抗体筛查,抗体阳性者进一步检测血小板抗-人类白细胞抗原(HLA)与抗-人类血小板抗原(HPA),分别按有无输血史、有无既往妊娠史、民族、孕周进行分组,分析妊娠期妇女PLT抗体阳性的影响因素。结果 2 790例妊娠期妇女中检出PLT抗体367例(总阳性率13.15%),其中抗-HLA、抗-HPA和抗-HLA+抗-HPA占比分别为47.96%、39.24%和12.80%;有无输血史组间、有无既往妊娠史组间PLT抗体总阳性率分别比较,差异有统计学意义(P<0.05);汉族与少数民族组间PLT抗体总阳性率比较,差异无统计学意义(P>0.05);按孕周分组发现,随着孕周增加,抗-HLA阳性率随之升高(P<0.05),且有9例孕早期PLT抗体阴性孕妇,孕晚期PLT抗体转阳;其中输血史和既往妊娠史是PLT抗体产生的危险性因素。结论 妊娠期妇女PLT抗体阳性分型结果以抗-HLA为主,PLT血型抗体的产生与既往妊娠史和输血史密切相关,根据输血史和妊娠史,可对妊娠期妇女PLT抗体进行更有效的动态监测。
Objective The expression of platelet(PLT)antibody in the serum of pregnant women was detected and the related influencing factors of PLT antibody positive in this population were analyzed.Methods A total of 2,790 pregnant women were selected as the study subjects,and PLT antibody screening was performed by solid phase erythrocyte agglutination method.Platelet anti-human leukocyte antigen(HLA)and anti-human platelet antigen(HPA)were further detected among the antibody-positive women,and they were grouped according to blood transfusion history,previous pregnancy history,ethnicity,and gestational age respectively to evaluate the effect of PLT antibody positive in pregnant women.Results Among 2,790 pregnant women,367 cases(positive rate 13.15%)of PLT antibodies were detected,among which anti-HLA,anti-HPA and anti-HLA+anti-HPA accounted for 47.96%,39.24%,and 12.80%,respectively;the total positive rate of PLT antibody was compared between groups with or without blood transfusion history,and the difference was statistically significant(P<0.05).There was statistical significance(P<0.05)in the total positive rate of PLT antibody between groups with or without previous pregnancy history;there was no significant difference(P>0.05)in the total positive rate of PLT antibody between Han and minority groups.Grouping by gestational age,it was found that as gestational age increased,the positivity rate of anti HLA increased(P<0.05),and 9 pregnant women with negative PLT antibodies in early pregnancy and positive PLT antibodies in late pregnancy were also found.The history of blood transfusion and previous pregnancy are risk factors for its occurrence.Conclusion The positive typing results of PLT antibodies in pregnant women are mainly anti-HLA,and the production of PLT blood group antibodies is closely related to the history of pregnancy and blood transfusion.According to the history of blood transfusion and pregnancy history,the dynamic monitoring of PLT antibodies in pregnant women can be more effective.
作者
涂飞
毕雅坤
王应辉
杨永莲
左丽
TU Fei;BI Yakun;WANG Yinghui;YANG Yonglian;ZUO Li(School of Basic Medicine,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Blood Transfusion,Guiyang Maternal and Child Health Care Hospital,Guiyang 550003,Guizhou,China;School of Clinical Laboratory Science,Guizhou Medical University,Guiyang 550004,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2024年第2期261-266,共6页
Journal of Guizhou Medical University
基金
贵州省科技项目(黔科合作基础-ZK[2023]一般009)。
关键词
血小板抗体检测
HLA抗体
HPA抗体
孕妇
民族
输血史
妊娠史
孕周
platelet antibody detection
anti-HLA
anti-HPA
pregnant women
nation
history of blood transfusion
history of pregnancy
gestational age
