摘要
陀螺果(Melliodendron xylocarpum)是中国特有的落叶乔木,具备较高的观赏、油用、药用和材用价值,但目前其种质资源已处于濒危状态。本研究以陀螺果当年生幼嫩茎段为材料,围绕启动培养、增殖培养、生根培养以及炼苗移栽四个阶段展开研究,构建陀螺果离体快繁技术体系。结果表明:启动培养阶段,75%乙醇浸泡30 s与0.1%HgCl_(2)浸泡12 min结合消毒处理污染率最低,其中真菌污染率11.11%,细菌污染率2.22%,诱导率为69.48%,存活率为80.00%,能够达到陀螺果茎段外植体表面消毒的目的;增殖培养阶段,1.0~1.5 mg·L^(-1)BA可以显著促进陀螺果试管苗的增殖和生长,增殖系数最高为4.53~4.70;生根培养阶段,2.0 mg·L^(-1)IAA能够有效诱导陀螺果试管苗生根,生根率87.23%,且移栽成活率最高,为88.33%,成苗率达77.05%。本研究首次构建了高效的陀螺果离体快繁技术体系,研究结果能够为其快速大量繁殖和产业化推广提供技术支撑。
Melliodendron xylocarpum,a deciduous tree endemic to China,possesses exceptional ornamental,oil,medicinal and timber value,but its genetic resources are currently endangered.This study employed young stem segments of M.xylocarpum as the primary material and conducted research across four phases including initiation culture,multiplication culture,rooting culture and transplanting in order to establish an in vitro rapid propagation technology system of M.xylocarpum.The results showed that the combination of 75%ethanol immersion for 30 s and 0.1%HgCl_(2) immersion for 12 min at the initiation culture stage had the lowest contamination rates with fungal contamination rate of 11.11%and bacterial contamination rate of 2.22%,and the highest induction rate was 69.48%and survival rate was 80.00%,which were able to effectively sterilize the surface of M.xylocarpum stem segments explants.During the multiplication culture stage,1.0-1.5 mg·L^(-1) BA significantly promoted the growth of in vitro shoots with the highest multiplication rate of 4.53-4.70.Additionally,2.0 mg·L^(-1) IAA was effective in promoting root induction of in vitro shoots with rooting rate of 87.23%and had the highest transplanting survival rate of 88.33%with 77.05%seedling rate in the rooting culture phase.This study has pioneered a highly efficient in vitro rapid propagation technology for M.xylocarpum,providing crucial technical support for its large-scale propagation and industrialization promotion.
作者
顾怡婷
潘月
龙超霞
冷一凡
黄远祥
高平
冯蘖
蒋凭宏
文书生
GU Yiting;PAN Yue;LONG Chaoxia;LENG Yifan;HUANG Yuanxiang;GAO Ping;FENG Nie;JIANG Pinghong;WEN Shusheng(College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China;Sichuan Tianyi Ecological Garden Group Co.,Ltd.,Chengdu 611130,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第1期177-184,共8页
Plant Physiology Journal
基金
四川天艺生态园林集团股份有限公司项目(TYST202101)
江苏高校优势学科建设工程(PAPD)
南京林业大学大学生创新训练计划项目(2022NFUSPITP0391)。
关键词
陀螺果
离体快繁
增殖
生根
炼苗移栽
Melliodendron xylocarpum
in vitro rapid propagation
multiplication
rooting
acclimatization and transplanting
