摘要
目的:探讨抗氧化相关长链非编码RNAs(lncRNAs)预后风险评分模型对胃癌患者预后的判断价值以及与免疫微环境的关系。方法:通过TCGA数据库下载胃癌转录组数据和临床信息。通过lncRNAs和抗氧化基因的共表达分析得到抗氧化相关lncRNAs。使用单因素cox回归分析和lasso回归分析筛选并构建风险评分。采用Log-Rank检验比较两组间的生存差异。应用受试者工作特征(ROC)曲线评估预后风险模型对患者预后判断的特异性及敏感度。结合风险评分和临床参数构建列线图。TIMER2.0在线评估每个样本的免疫细胞浸润情况。TIDE网站在线分析每个样本对免疫治疗敏感性。结果:通过单因素cox回归分析和lasso回归分析构建了一个包括12个lncRNAs的风险评分。风险评分是患者预后的独立影响因素[HR=5.406(3.131~9.335),P<0.001]。风险评分与多种抑制性免疫细胞浸润呈正相关(M2型巨噬细胞、肿瘤相关成纤维细胞)。同时发现,高风险组存多种免疫检查点基因的异常表达,TIDE评分更高,提示高风险组对免疫治疗更敏感。结论:基于抗氧化相关lncRNAs风险评分和临床参数构建的列线图对胃癌患者预后具有良好的预测作用,风险评分可作为临床个体化免疫治疗的参考。
Objective:To investigate the value of antioxidant-associated long non-coding RNAs(lncRNAs)risk score model in prognosis and the association with immune microenvironment of the gastric cancer patients.Methods:Gastric cancer transcriptome data and clinical information were downloaded from TCGA database.Antioxidant-associated lncRNAs were obtained by co-expression analysis of lncRNAs and antioxidant genes.Risk score was constructed using univariate cox regression analysis and lasso regression analysis.Log-Rank test was used to compare the survival differences between two groups.Receiver operating characteristic curve(ROC)was used to assess the specificity and sensitivity of the prognostic risk score model.Nomogram was constructed combining risk score and clinical parameters.Immune cell infiltration was assessed by TIMER 2.0.Immunotherapy sensitivity of each sample was analyzed at TIDE website.Results:A risk score including 12 lncRNAs was constructed by univariate cox regression analysis and lasso regression analysis.The risk score was an independent factor influencing patient prognosis[HR=5.406(3.131~9.335),P<0.001].Risk score was positively correlated with multiple suppressive immune cells infiltration(M2 macrophage,tumor-associated fibroblast).Meanwhile,multiple aberrant expression of immune checkpoint genes and higher TIDE score were found in high-risk group,suggesting that high-risk groups may be more sensitive to immunotherapy.Conclusion:The antioxidant-associated lncRNAs risk score is a good prognostic predictor and can act as a reference in individualized immunotherapy for gastric cancer patients.
作者
张晓鑫
许紫鹏
陆烟生
程杰
朱征海
ZHANG Xiao-xin;XU Zi-peng;LU Yan-sheng;CHENG Jie;ZHU Zheng-hai(Department of General Surgery,Xishan People's Hospital of Wuxi City,Wuxi 214105,China)
出处
《中国现代普通外科进展》
CAS
2024年第2期122-128,共7页
Chinese Journal of Current Advances in General Surgery
基金
无锡市科技发展医疗卫生指导性计划项目(NZ2019033)。
