摘要
目的比较3种不同方案制备的动物模型,探索稳定、可靠且重复性好的小鼠慢性心力衰竭模型。方法将25只雄性C57BL/6J小鼠随机分为4组:正常组(ZC组)、实验A组(MA组)、B组(MB组)和C组(MC组)。实验组采取不同制备方案连续注射ISO,其中MA组和MB组为浓度递减造模法,MA组(第1天10 mg/kg、第2天5 mg/kg、第3~30天2.5 mg/(kg·d);皮下注射30 d);MB组(第1天20 mg/kg、第2天10 mg/kg、第3~14天5 mg/(kg·d);皮下注射14 d);MC组(浓度恒定7.5 mg/(kg·d),腹腔注射28 d),构建慢性心衰动物模型。在注射结束后的第2天,计算各组小鼠存活率和成模率情况。通过心脏超声检测心功能,并用ELISA测定血清中NT-pro BNP、IL-6、TNF-α水平。结果在第30天注射结束后,各实验组虽都能有效诱导慢性心力衰竭,但发现7.5 mg/kg浓度MC组的造模情况最稳定,更适合后续开展中医药相关的心衰研究。结论ISO制备小鼠慢性心衰模型以恒定7.5 mg/(kg·d),连续腹腔注射28 d为最佳方案。
Objective To compare animal models of chronic heart failure(CHF)prepared by three different protocols,to establish a stable,reliable,and reproducible mouse model of CHF.Methods Twenty-five male C57BL/6J mice were divided randomly into four groups:a blank group,model A group(MA group),model B group(MB group),and model C group(MC group).The model groups adopted different preparation protocols for continuous injection of isoprenaline.The MA group and MB group were dose-decreasing models:MA group:subcutaneous injection of 10 mg/kg on day 1,5 mg/kg on day 2,2.5 mg/(kg·d)on days 3~30,total 30 days;and MB group:subcutaneous injection of 20 mg/kg on day 1,10 mg/kg on day 2,5 mg/(kg·d)on days 3~14,total 14 days.The MC group used a constant dose of intraperitoneal injection of 7.5 mg/(kg·d)for 28 days.The day after the final injection,the survival and model-formation rates for each group of mice were calculated.Cardiac function was measured by cardiac ultrasound and serum levels of N-terminal pro B-type natriuretic peptide,interleukin-6,and tumor necrosis factor-αwere measured.Results CHF was successfully induced in all the model groups after all injections at the end of the fourth week.However,comprehensive test result showed that the MC model was the most stable.Conclusions An isoprenaline-induced mouse model of CHF using constant intraperitoneal injection of 7.5 mg/(kg·d)for 28 days may be the most suitable model for subsequent research on traditional Chinese medicine.
作者
谈宇权
张君宇
杨梦
王菲
钟森杰
李琳
胡志希
TAN Yuquan;ZHANG Junyu;YANG Meng;WANG Fei;ZHONG Senjie;LI Lin;HU Zhixi(Institute of Traditional Chinese Medicine Diagnosis,Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2024年第2期161-167,共7页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金(82274412)
湖南省自然科学基金(2020JJ4062,2020JJ5408)
湖南省教育厅项目(21A0230,21B0361)
湖南省学位与研究生教育教改项目(2020JGZX012)
湖南省教育改革课题(HNJG-2021-0594)。
