摘要
目的:探究槐耳多糖(HP)调节SPHK1/S1P/S1PR3信号通路对宫颈癌细胞恶性生物学行为的影响。方法:MTT检测槐耳多糖(0、25、50、100、200、400μg/mL)处理的宫颈癌细胞活力,筛选最佳药物浓度。实验分为对照组(Control组)、槐耳多糖低、中、高浓度组(HP-L、HP-M、HP-H组)和槐耳多糖高浓度+SphK1激活剂K6PC-5组(HP-H+K6PC-5组),观察细胞增殖、迁移和侵袭情况;western blot检测SPHK1、S1P、S1PR3、Snail、N-cadherin、E-cadherin蛋白水平。结果:处理24、48、72h后,与0μg/mL比较,50μg/mL、100μg/mL、200μg/mL和400μg/mL的HP处理的细胞活力显著降低(P<0.05)。HP-L组、HP-M组和HP-H组细胞Edu阳性率、侵袭细胞数、划痕愈合率及Snail、N-cadherin、SPHK1、S1P、S1PR3水平显著低于Control组(P<0.05),E-cadherin水平显著升高(P<0.05)。HP-H+K6PC-5组细胞Edu阳性率、侵袭细胞数、划痕愈合率及Snail、N-cadherin、SPHK1、S1P、S1PR3水平显著高于HP-H组(P<0.05),E-cadherin水平显著降低(P<0.05)。结论:HP可能通过抑制SPHK1/S1P/S1PR3信号通路抑制宫颈癌细胞的增殖、侵袭和迁移。
Objective:To investigate the impacts of Huaier polysaccharide(HP)regulating the SPHK1/S1P/S1PR3 signaling pathway on the malignant biological behavior of cervical cancer cells.Methods:MTT was used to detect the viability of cervical cancer cells treated with Sophora auricula polysaccharides(0,25,50,100,200,400μg/mL)and screen for the optimal drug concentration.The subjects were divided into control group,low,medium,and high concentration groups of Huaier polysaccharide(HP-L,HP-M,HP-H groups),and high concentration of Huaier polysaccharide+SphK1 activator K6PC-5 group(HP-H+K6PC-5 group).The cell proliferation,migration and invasion were observed;Western blot was used to detect the protein levels of SPHK1,S1P,S1PR3,Snail,N-cadherin,and E-cadherin.Results:After treatment for 24,48 and 72h,HP treatments of 50μg/mL,100μg/mL,200μg/mL and 400μg/mL significantly decreased cell viability compared with 0μg/mL(P<0.05),Edu positive rate,number of invasive cells,scratch healing rate and Snail,N-cadherin,SPHK1,S1P and S1PR3 levels in HP-L,HP-M and HP-H groups were obviously lower than those in Control group(P<0.05),E-cadherin levels significantly increase(P<0.05).The Edu positive rate,number of invasive cells,scratch healing rate and Snail,N-cadherin,SPHK1,S1P and S1PR3 levels in HP-H+K6PC-5 group were obviously higher than those in HP-H group(P<0.05),E-cadherin levels significantly decrease(P<0.05).Conclusion:HP may inhibit the proliferation,invasion and migration of cervical cancer cells by inhibiting SPHK1/S1P/S1PR3 signaling pathway.
作者
李丽品
马素艳
安入征
LI Lipin;MA Suyan;AN Ruzheng(Shijiazhuang Ping'an Hospital,Hebei Shijiazhuang 050000,China)
出处
《河北医学》
CAS
2024年第3期411-416,共6页
Hebei Medicine
基金
河北省中医药管理局科研计划项目,(编号:2010132)。
