摘要
目的评估半巢式全自动实时荧光定量PCR检测与涂片抗酸染色法在活动性肺结核早期检验中的应用效能。方法选取2022年9月—2023年9月于平邑县结核病防治所就诊的90例疑似肺结核患者为研究对象,采用Xpert MTB/RIF技术、涂片抗酸染色法检验及简单法固体培养法同时检测90例疑似肺结核患者痰标本,对检测结果进行比较。结果以固体培养结果作为金标准,痰培养阳性率85.56%,阴性率14.44%。Xpert MTB/RIF阳性率84.44%、阴性率15.56%。涂片抗酸染色法检验阳性率48.88%、阴性率51.11%。Xpert MTB/RIF检验的灵敏度97.40%、准确度96.67%、阳性预测值98.68%以及阴性预测值85.71%均高于涂片抗酸染色法检验50.64%、52.22%、88.64%、17.39%,差异有统计学意义(χ^(2)=43.768、46.722、5.922、22.546,P均<0.05)。结论与涂片抗酸染色法相比,在活动性肺结核早期检验中采用半巢式全自动实时荧光定量PCR检测(Xpert MTB/RIF)的应用效能更高。
Objective To evaluate the efficacy of the application of semi-nested fully automated real-time fluorescent quantitative PCR assay with smear antacid staining method in the early detection of active pulmonary tuberculosis.Methods 90 patients with suspected pulmonary tuberculosis attending the Pingyi County Tuberculosis Control Center between September 2022 and September 2023 were included as the study objects.The sputum specimens of 90 sus⁃pected TB patients were tested simultaneously by Xpert MTB/RIF technique,smear antacid staining test and simple method solid culture method,and the test results were compared.Results Using the solid culture results as the gold standard,the sputum culture positive rate was 85.56%and the negative rate was 14.44%.The Xpert MTB/RIF posi⁃tive rate was 84.44%and the negative rate was 15.56%.Smear antacid staining test positive rate 48.88%,negative rate 51.11%.The Xpert MTB/RIF test had a higher sensitivity of 97.40%,accuracy of 96.67%,positive predictive value of 98.68%,and negative predictive value of 85.71%than the smear antacid staining method test of 50.64%,52.22%,88.64%,and 17.39%,the differences were statistically significant(χ^(2)=43.768,46.722,5.922,22.546,all P<0.05).Conclusion Compared with the smear antacid staining method,the application of semi-nested fully automated real-time fluorescence quantitative PCR assay(Xpert MTB/RIF)in the early detection of active tuberculosis is more efficacious.
作者
咸丽
XIAN Li(Department of Laboratory,Pingyi County Tuberculosis Control Center,Linyi,Shandong Province,273300 China)
出处
《系统医学》
2024年第2期87-89,109,共4页
Systems Medicine
