摘要
目的 分析化学发光免疫测定法(CLIA)、快速血清反应素试验(RPR)、梅毒螺旋体明胶颗粒凝集试验(TPPA)检测血清中梅毒螺旋体抗体的结果。方法 100例疑似梅毒患者血清样本为研究对象,均采用CLIA、RPR、TPPA进行检测,并以重组免疫印迹法(RIBA)检测结果为金标准,分析三种检测方式的检测结果 ,并比较三种检测方式的诊断效能。结果 CLIA检出阳性59例,阴性41例。RPR检出阳性73例,阴性27例。TPPA检出阳性59例,阴性41例。CLIA诊断准确率为69.00%(69/100),敏感度为68.92%(51/74),特异度为69.23%(18/26);RPR诊断准确率为97.00%(97/100),敏感度为97.30%(72/74),特异度为96.15%(25/26);TPPA诊断准确率为77.00%(77/100),敏感度为74.32%(55/74),特异度为84.62%(22/26)。RPR的诊断准确率、敏感度显著高于CLIA、TPPA(P<0.05);RPR的特异度高于CLIA(P<0.05);CLIA与TPPA的诊断准确率、敏感度、特异度差异较小(P>0.05)。结论 RPR在梅毒螺旋体抗体的临床诊断中具有更佳的诊断准确率,可为梅毒患者的治疗提供可靠的诊疗依据。
Objective To analyze the results of detection of Treponema pallidum antibody in serum by chemiluminescent immunoassay(CLIA),rapid plasma reagin(RPR)and Treponema pallidum partical assay(TPPA).Methods 100 serum samples from suspected syphilis patients were selected for study.All samples were tested by CLIA,RPR,and TPPA method,and the results of recombinant immunoblot assay(RIBA)were used as the gold standard.The results of the three detection methods were analyzed,and the diagnostic efficiency of the three detection methods was compared.Results CLIA detected 59 positive cases and 41 negative cases;RPR detected 73 positive cases and 27 negative cases;TPPA detected 59 positive cases and 41 negative cases.The diagnostic accuracy of CLIA was 69.00%(69/100),the sensitivity was 68.92%(51/74),and the specificity was 69.23%(18/26).The diagnostic accuracy of RPR was 97.00%(97/100),the sensitivity was 97.30%(72/74),and the specificity was 96.15%(25/26).The diagnostic accuracy of TPPA was 77.00%(77/100),the sensitivity was 74.32%(55/74),and the specificity was 84.62%(22/26).The diagnostic accuracy and sensitivity of CLIA were significantly higher than that of CLIA and TPPA(P<0.05).The specificity of RPR was higher than CLIA(P<0.05).The difference of diagnostic accuracy and sensitivity between CLIA and TPPA was small(P>0.05).The difference in diagnostic accuracy,sensitivity and specificity between CLIA and TPPA was small(P>0.05).Conclusion RPR method has better diagnostic accuracy in the clinical diagnosis of Treponema pallidum antibody,and can be used to provide reliable diagnosis and treatment basis for the treatment of patients with syphilis.
作者
赵颖
马跃
ZHAO Ying;MA Yue(Tiexi District Center for Disease Control and Prevention,Shenyang 110000,China)
出处
《中国现代药物应用》
2024年第5期72-74,共3页
Chinese Journal of Modern Drug Application
