摘要
目的:优化紫花地丁总黄酮的内部沸腾法提取工艺,提高总黄酮提取率。方法:考察提取过程中涉及到的7个因素对提取率的影响,即解吸附溶剂浓度、解吸附溶剂用量、解吸附所用时间、提取溶剂浓度、提取温度、提取时间和提取溶剂用量,并在单因素试验基础上,采用Box-Benhnken试验和响应面分析法,优化了紫花地丁总黄酮的提取工艺。结果:先用浓度86%的C_(2)H_(5)OH溶液3 mL/g解吸附20 min,然后用浓度22%C_(2)H_(5)OH溶液25 mL/g在86℃提取紫花地丁2次,每次5 min,结果紫花地丁总黄酮提取得率为13.5%。结论:内部沸腾提取法快速、有效且经济,用于紫花地丁中总黄酮的提取效果良好。
Objective:To optimize the extraction technology of the total flavonoids in Viola yedoensis Makinoby using inner ebullitionand and improve the extraction yield.Methods:Single factor experiments were conducted to investigate the effects of seven factors on the extraction,including the concentration and the amount of desorption agent,desorption time,the concentration and the amount of extractant,extraction temperature and time.Through Box-Behnken response surface method,the optimal extraction process was optimized.Results:It was found that when Viola yedoensis Makinowere desorbed 20 minutes using 86%ethanol 3 mL/g and then extracted twice(5 min/time)in 22%ethanol 25 mL/g under 86℃,the yield of total flavonoids of 13.5%was obtained.Conclusion:Inner ebullition extraction was rapid,effective and economic in the extraction of total flavonoids from Viola yedoensis Makino.
作者
段建荣
李春梅
高晓莉
胡雪娇
陈卓
Duan Jianrong;Li Chunmei;Gao Xiaoli;HuXuejiao;Chen Zhuo(Pharmacy Department,Shalingzi Hospital,Zhangjiakou 075000,China)
出处
《广东化工》
CAS
2024年第2期42-44,22,共4页
Guangdong Chemical Industry
基金
张家口市科技计划项目(2221215D)。
关键词
内部沸腾
紫花地丁
提取
总黄酮
工艺优化
inner ebullition
viola yedoensis makino
extraction
total flavonoids
process optimization
