摘要
目的 探讨转录激活因子6(activating transcription factor 6,ATF6)和干扰素α(interferon α,IFN-α)在喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)组织中的表达及意义。方法 选取2015年3月~2020年3月于河南科技大学临床医学院/河南科技大学第一附属医院入院治疗的100例LSCC患者,收集整理其肿瘤部位、分化程度、淋巴结转移等临床病理特征;采用免疫组织化学法检测组织中ATF6和IFN-α的表达;采用Spearman法分析LSCC组织中ATF6与IFN-α表达的相关性;用Kaplan-Meier法分析LSCC组织中ATF6,IFN-α表达与患者三年生存率的关系;采用COX回归分析LSCC患者一年死亡的影响因素。结果 ATF6在LSCC组织中阳性率(76.00%)明显高于癌旁正常组织(13.00%),IFN-α在LSCC组织中阳性率(29.00%)明显低于癌旁正常组织(74.00%),差异具有统计学意义(χ^(2)=80.352,40.536,均P<0.05);TNM分期为Ⅲ+Ⅳ期、浸润深度为深层、发生淋巴结转移的LSCC患者ATF6阳性表达比例均显著高于TNM分期为Ⅰ+Ⅱ期、浸润深度为浅层、未发生淋巴结转移的LSCC患者(χ^(2)=7.310,9.223,5.123,均P<0.05)。TNM分期为Ⅲ+Ⅳ期、浸润深度为深层、发生淋巴结转移的LSCC患者IFN-α阴性表达比例均显著高于TNM分期为Ⅰ+Ⅱ期、浸润深度为浅层、未发生淋巴结转移的LSCC患者(χ^(2)=8.564,5.021,5.203,均P<0.05);LSCC组织中ATF6与IFN-α表达具有负相关性(r=-0.415,P<0.05);ATF6阳性表达组LSCC患者三年生存率(50.00%)显著低于ATF6阴性表达组(83.33%),IFN-α阳性表达组LSCC患者三年生存率(82.76%)显著高于IFN-α阴性表达组(47.89%)(Log rank χ^(2)=8.002,10.854,均P<0.05)。ATF6(HR=1.735,95%CI:1.159~2.598),IFN-α(HR=0.624,95%CI:0.439~0.886)均是LSCC患者死亡的影响因素(P<0.05)。结论 LSCC组织中ATF6阳性表达率升高、IFN-α阳性表达率下降,均与患者临床病理特征及预后密切相关。
Objective To investigate the expression of activating transcription factor 6(ATF6)and interferonα(IFN-α)and their significance in laryngeal squamous cell carcinoma(LSCC)tissue.Methods A total of 100 LSCC patients admitted to Clinical Medical College of Henan University of Science and Technology/the First Affiliated Hospital of Henan University of Science and Technology from March 2015 to March 2020 were selected,and their clinicopathological features such as tumor location,degree of differentiation,and lymph node metastasis were collected and organized.Immunohistochemical method was applied to detect the expression of ATF6 and IFN-αin tissues.Spearman method was used to analyze the correlation between ATF6 and IFN-αexpression in LSCC tissue.Kaplan-Meier method was applied to analyze the relationship between ATF6 and IFN-αexpression in LSCC tissue and 3-year survival rate of patients.Cox regression was used to analyze the influencing factors of 3-year mortality in LSCC patients.Results The positive rate of ATF6 in LSCC tissue(76.00%)was higher than that in normal tissues adjacent to cancer(13.00%),the positive rate of IFN-αin LSCC tissue(29.00%)was lower than that in normal tissues adjacent to cancer(74.00%),and the difference was statistically significant(χ^(2)=80.352,40.536,all P<0.05).The proportions of ATF6 positive expression in LSCC patients with TNM stage III+IV,deep infiltration depth,and lymph node metastasis were significantly higher than those in LSCC patients with TNM stage I+II,shallow infiltration depth,and no lymph node metastasis(χ^(2)=7.310,9.223,5.123,all P<0.05).The proportions of IFN-αnegative expression in LSCC patients with TNM stage III+IV,deep infiltration depth,and lymph node metastasis were significantly higher than those in LSCC patients with TNM stage I+II,shallow infiltration depth,and no lymph node metastasis(χ^(2)=8.564,5.021,5.203,all P<0.05).There was a negative correlation between ATF6 and IFN-αexpression in LSCC tissues(r=-0.415,P<0.05).The 3-year survival rate of LSCC patients in the ATF6 positive expression group(50.00%)was significantly lower than that in the ATF6 negative expression group(83.33%),while the 3-year survival rate of LSCC patients in the IFN-αpositive expression group(82.76%)was significantly higher than that in the IFN-αnegative expression group(47.89%)(Log rankχ^(2)=8.002,10.854,all P<0.05).ATF6(HR=1.735,95%CI:1.159~2.598)and IFN-α(HR=0.624,95%CI:0.439~0.886)were influencing factors for the mortality of LSCC patients.Conclusion The positive expression rate of ATF6 increased and the positive expression rate of IFN-αdecreased in LSCC tissues.They were closely related to the clinical pathological characteristics and prognosis of patients.
作者
席恺
张苗苗
张曦
张腾腾
邢丙文
XI Kai;ZHANG Miaomiao;ZHANG Xi;ZHANG Tengteng;XING Bingwen(Department of Otolaryngology,Clinical Medical College of Henan University of Science and Technology/the First Affiliated Hospital of Henan University of Science and Technology,Luoyang,Henan Luoyang,471003,China)
出处
《现代检验医学杂志》
CAS
2024年第2期12-17,共6页
Journal of Modern Laboratory Medicine
基金
河南省医学科技攻关计划项目(LHGJ20220680):不同前庭康复时机在前庭神经炎急性期患者中的疗效分析。
关键词
喉鳞状细胞癌
转录激活因子6
干扰素Α
淋巴结转移
浸润深度
内质网应激
laryngeal squamous cell carcinoma
activating transcription factor 6
interferonα
lymph node metastasis
infiltration depth
endoplasmic reticulum stress
