摘要
目的探讨大麻二酚(cannabidiol,CBD)对刀豆蛋白A(concanavalin A,ConA)诱导小鼠急性免疫性肝炎中肝脏巨噬细胞的影响。方法30只C57BL/6雄性小鼠随机分为正常对照组、ConA模型组、CBD实验组(低剂量5 mg/kg、中剂量15 mg/kg、高剂量30 mg/kg)。实验结束后称取小鼠肝重,取外周血测定ALT和AST水平,ELISA法测定肝内细胞因子TNF-α、IL-6、TGF-β和IL-10含量,流式细胞术测定肝脏中巨噬细胞的分型和比例。结果ConA模型组小鼠肝重较正常对照组减轻,血清ALT和AST水平升高(P<0.01),肝内细胞因子TNF-α和IL-6水平升高,TGF-β和IL-10水平降低(P<0.05),同时M1型巨噬细胞比例升高,M2型巨噬细胞比例下降(P<0.05)。与ConA模型组相比,CBD实验组小鼠肝重有不同程度的增加,血清ALT和AST水平下降,以中、高剂量组下降更显著(P<0.01和P<0.05),同时小鼠肝内TNF-α和IL-6水平下降,TGF-β和IL-10水平升高,中、高剂量组TNF-α、IL-6下降以及TGF-β升高更显著(P<0.05)。另外,低、中剂量CBD实验组M1型巨噬细胞比例下降,M2型巨噬细胞比例升高,以中剂量实验组M2型巨噬细胞升高更显著(P<0.05)。结论CBD可以通过促进肝脏中巨噬细胞M1向M2极化来缓解ConA诱导的小鼠急性免疫性肝损伤。
Objective To investigate the effect of cannabidiol(CBD)on hepatic macrophages in mice with acute immune hepatitis induced by concanavalin A(ConA).Methods Thirty male C57BL/6 mice were randomly divided into normal control group,ConA model group and CBD experimental group(low dose 5 mg/kg,medium dose 15 mg/kg,high dose 30 mg/kg).After the experiment,the liver weight of mice was measured.ALT and AST levels were measured in peripheral blood,the contents of TNF-α,IL-6,TGF-βand IL-10 in liver were determined by ELISA,and the types and proportions of macrophages in liver were determined by flow cytometry.Results Compared with the normal control group,the liver weight of mice in ConA model group was decreased.The serum ALT and AST levels were increased(P<0.01).The liver cytokines TNF-αand IL-6 levels were increased,the levels of TGF-βand IL-10 were decreased(P<0.05).The proportion of M1-type macrophages was increased,while the proportion of M2-type macrophages was decreased(P<0.05).Compared with the ConA model group,the liver weight of mice in the CBD experimental group increased to different degrees,and the serum ALT,AST levels decreased.The decrease was more significant in the medium and high dose groups(P<0.01 and P<0.05).Meanwhile,the levels of TNF-α,IL-6 in the liver of mice were decreased,and the levels of TGF-β,IL-10 were increased.The decrease of TNF-α,IL-6 and the increase of TGF-βin the middle and high dose groups were more significant(P<0.05).In addition,the proportion of M1-type macrophages decreased and the proportion of M2-type macrophages increased in the low-dose and medium-dose CBD groups,and the increase of M2-type macrophages was more significant in the medium-dose group(P<0.05).Conclusion CBD can alleviate ConA-induced acute immune liver injury in mice by promoting the polarization of macrophages M1 to M2 in mice liver.
作者
韩冰
任建峰
涂传涛
HAN Bing;REN Jianfeng;TU Chuantao(Department of Gastroenterology and Hepatology,Shanghai Fourth People′s Hospital Affiliated to Tongji University,Shanghai 200434;Department of Gastroenterology,Shanghai Public Health Clinical Center,Fudan University,China)
出处
《胃肠病学和肝病学杂志》
CAS
2024年第3期257-260,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
上海市虹口区卫生健康委员会科研课题面上项目(虹卫2002-16)。
