摘要
目的:初步探讨糖尿病肾病(DKD)小鼠CD4^(+)T免疫细胞在脂质组学方面的差异,筛选出具有生物学意义的差异代谢产物。方法:先采用CD4 (L3T4) MicroBeads免疫磁珠法分离BKS.Cg-Dock7^(m+/+)Lepr^(db)/J自发性DKD小鼠脾脏CD4^(+)T免疫细胞;流式细胞术鉴定CD4^(+)T免疫细胞纯度,LC-MS/MS技术检测CD4^(+)T免疫细胞非靶向脂质组学,对差异代谢产物进行分析。结果:LC-MS法检测出463个代谢产物;PCA和OPLS-DA分析显示代谢组分明显分离;筛选出24种差异代谢物。KEGG及富集分析可知差异代谢物涉及甘油磷脂代谢紊乱。结论:CD4^(+)T细胞磷脂类代谢与DKD的发生密切相关,靶向DKD CD4^(+)T细胞的磷脂类代谢可能是DKD治疗的新方向。
Objective:To investigate the lipidomics differences of CD4^(+)T immune cells in diabetic kidney disease(DKD)mice,and screen out the differential metabolites with biological significance.Methods:CD4(L3T4)MicroBeads immunomagnetic beads were used to isolate CD4^(+)T immune cells from spleen of BKS.Cg-Dock7^(m+/+)Lepr^(db)/J mice with spontaneous DKD;the purity of CD4^(+)T cells were identified by flow cytometry.The non-targeted lipidomics of CD4^(+)T cells were detected by LC-MS/MS,and the differ-ential metabolites were analyzed.Results:A total of 463 metabolites were detected by LC-MS.PCA and OPLS-DA analysis showed that the metabolic components were significantly separated;twenty-four differential metabolites were screened out.KEGG and enrich-ment analysis showed that the differential metabolites involved in the disorder of glycerol phospholipid metabolism.Conclusion:Phos-pholipid metabolism of CD4^(+)T cells is closely related to the occurrence of DKD.Phospholipid metabolism targeting DKD CD4^(+)T cells in DKD may be a new direction of DKD treatment.
作者
张小雨
谭海波
黄敏仪
贝伟剑
杨祎琦
ZHANG Xiaoyu;TAN Haibo;HUANG Minyi;BEI Weijian;YANG Yiqi(Institute of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《中国免疫学杂志》
CAS
CSCD
2024年第3期471-477,共7页
Chinese Journal of Immunology
基金
广东省医学科学技术研究基金项目(B2020023)。
