摘要
目的 建立羌活胜湿汤(Qianghuo Shengshi decoction, QSD)物质基准的指纹图谱及多指标成分含量测定方法,研究QSD物质基准量值传递规律,为QSD后续开发及质量控制评价提供依据。方法 依据古籍记载制备15批QSD物质基准样品溶液,测定其出膏率,建立指纹图谱并进行相似度评价,明确特征峰及特征峰归属,完成QSD物质基准中8个指标成分的含量测定及饮片-物质基准量值传递分析。结果 15批QSD物质基准指纹图谱相似度处于0.882~0.938之间,共确定24个共有峰,并指认出8个共有峰分别为绿原酸、升麻素苷、阿魏酸、洋川芎内脂Ⅰ、5-O-甲基维斯阿米醇苷、甘草酸铵、羌活醇、蛇床子素。15批QSD物质基准的出膏率为22.54%~29.92%,平均出膏转移率为70.69%。各指标性成分的含量及饮片-基准样品的平均转移率分别为绿原酸0.077 2%~0.106 6%和128.05%;升麻素苷0.015 5%~0.023 8%和120.26%;阿魏酸0.047 5%~0.076 0%和114.49%;洋川芎内脂Ⅰ0.006 6%~0.008 9%和132.93%;5-O-甲基维斯阿米醇苷0.007 2%~0.009 9%和90.27%;甘草酸铵0.181 6%~0.273 5%和80.53%;羌活醇0.000 7%~0.001 3%和50.97%;蛇床子素0.000 9%~0.002 2%和13.67%。结论 本实验采用指纹图谱、出膏率及多指标成分含量测定相结合的方法,对QSD饮片-物质基准的全过程进行量值传递分析,为该方的质量控制及复方制剂的开发提供参考。
OBJECTIVE To establish the fingerprint of the substance benchmark of Qianghuo shengshi decoction(QSD)and the determination method of multi-index composition content,study the transfer rule of the substance benchmark value of QSD,and provide a scientific basis for the subsequent development of QSD and quality control evaluation.METHODS Fifteen batches of QSD substance benchmark samples were prepared according to the method recorded in ancient medical books,and their fingerprint was established and evaluated,its characteristic peaks was clarified and assigned to the characteristic peaks,and we complete the content determination and transfer rate calculation of the 8 index components in the QSD substance benchmark.RESULTS The similarity of the fingerprint of 15 batches of QSD substance benchmark was between 0.882 and 0.938.A total of 24 common peaks were identified,and 8 common peaks were identified as chlorogenic acid,prim-O-glucosylcimifugin,ferulic acid,senkyunolide I,5-O-methylvisammioside,ammonium glycyrrhizinate,notopterygol,osthole.The paste yield of 15 batches of QSD was 22.54%-29.92%,and the average paste transfer rate was 70.69%.The content and average transfer rate of each index component from decoction pieces to substance benchmarks were 0.0772%-0.1066%and 128.05%for chlorogenic acid,prim-O-glucosylcimifugin 0.0155%-0.0238%and 120.26%,ferulic acid 0.0475%-0.0760%and 114.49%,senkyunolide I 0.0066%-0.0089%and 132.93%,5-O-methylvisammioside 0.0072%-0.0099%and 90.27%,ammonium glycyrrhizinate 0.1816%-0.2735%and 80.53%,notopterygol 0.0007%-0.0013%and 50.97%,osthole 0.0009%-0.0022%and 13.67%.CONCLUSION In this experiment,a combination of fingerprint,paste yield and multi-index composition content determination could be used to analysis the transfer rate of QSD decoction pieces to substance benchmarks,and provide a method reference for the quality control and subsequent development of this prescription.
作者
祁庆瑞
翟秉涛
岳宝森
赵锋
田欢
QI Qingrui;ZHAI Bingtao;YUE Baosen;ZHAO Feng;TIAN Huan(Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,Shaanxi University of Chinese Medicine,Xi'an 712046,China;Department of Pharmacy,Xi'an Hospital of Traditional Chinese Medicine,Xi'an 710021,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2024年第2期128-135,共8页
Chinese Pharmaceutical Journal
基金
陕西省中医药管理局重点科学研究项目资助(2021-02-22-010)
陕西省自然科学基础研究计划项目资助(2022JQ-932)
西安市科技计划项目资助(23YXYJ0042)
陕西省中药基础与新药研究重点实验室开放课题资助(KF202302)。
关键词
羌活胜湿汤
物质基准
指纹图谱
量值传递
Qianghuo Shengshi decoction
substance benchmark
fingerprint
quantitative transmitting
