摘要
目的研究电针经穴对脑出血后偏瘫痉挛大鼠脊髓颈膨大处谷氨酸受体2(GluR2)表达的影响,探讨电针缓解脑出血后偏瘫痉挛症状的机制。方法将45只健康SD大鼠随机分为假手术组9只、模型组18只、电针组18只。模型组和电针组大鼠采用立体定向在右侧内囊注入自体尾血方法建立脑出血模型,假手术组大鼠不注射自体尾血。造模后电针组采用电针刺激大鼠曲池与足三里穴,假手术组与模型组不给予任何干预。术后第3天、第7天对大鼠分别进行行为学检测[神经功能状态(采用Zea-longa评分评定)、肌张力(采用改良的Ashworth分级评分测定)、旷场实验],并采用Western blot法和免疫组化法检测脊髓颈膨大处GluR2表达情况。结果术后第3天、第7天与假手术组比较,模型组大鼠的Zea-longa评分和左前肢、左后肢的改良Ashworth评分均明显增高(P均<0.05),运动总路程明显缩短(P均<0.05),平均速度明显减慢(P均<0.05),静止时间明显延长(P均<0.05),脊髓颈膨大处GluR2蛋白相对表达量和阳性表达平均光密度值均明显降低(P均<0.05)。与模型组比较,电针组大鼠的Zea-longa评分和左前肢、左后肢的改良Ashworth评分均明显降低(P均<0.05),运动总路程明显延长(P均<0.05),平均速度明显加快(P均<0.05),静止时间明显缩短(P均<0.05),脊髓颈膨大处GluR2蛋白相对表达量和阳性表达平均光密度值均明显增高(P均<0.05)。结论电针刺激曲池和足三里穴可以显著改善脑出血大鼠痉挛性瘫痪症状,缓解肌张力增高,上调脊髓颈膨大处GluR2表达可能是电针治疗缓解脑出血后肢体痉挛的机制之一。
Objective It is to study the effect of electro-acupuncture on the expression of glutamate receptor 2(GluR2)in cervical enlargement of spinal cord in rats with hemiplegic spasm after cerebral hemorrhage,and to explore the mechanism of electroacupuncture in relieving hemiplegic spasm symptoms after cerebral hemorrhage.Methods Forty-five healthy SD rats were randomly divided into 9 rats in the sham operation group,18 rats in the model group and 18 rats in the electro-acupuncture group.The rats in the model group and electroacupuncture group were injected with autologous tail blood into the right internal capsule by stereotactic device to establish models of cerebral hemorrhage,and the rats in the sham operation group was not injected with autologous tail blood.After modeling,the rats of the electro-acupuncture group were stimulated with electro-acupuncture at Quchi and Zusanli acupoints,while the sham operation group and model group were not given any intervention.Behavioral tests[neurological status(assessed by Zea-longa score),muscle tension(assessed by modified Ashworth grading scale),and open field test]were performed in the rats on the 3rd and 7th days after surgery,and the expression of GluR2 in cervical enlargement of spinal cord was detected by Western blot and immunohistochemistry,respectively.Results Compared with the sham operation group on postoperative days 3 and 7,the Zea-longa scores and modified Ashworth scores of the left forelimb and left hindlimb of the rats in the model group were significantly increased(all P<0.05),the total distance traveled for locomotion was significantly shortened(P<0.05),the mean velocity was significantly slowed(P<0.05),the resting time was significantly increased(P<0.05),and the expression of GluR2 in cervical enlargement of spinal cord was significantly reduced(P<0.05).Compared with the model group,the Zea-longa scores and modified Ashworth scores of the left forelimb and left hindlimb of the rats in the electro-acupuncture group were significantly decreased(all P<0.05),the total distance traveled for locomotion was significantly increased(P<0.05),the mean velocity was significantly increased(P<0.05),the resting time was significantly decreased(P<0.05),and the expression of GluR2 in cervical enlargement of spinal cord was significantly increased(P<0.05).Conclusion Electro-acupuncture stimulation at“Quchi”and“Zusanli”can significantly improve the spastic paralysis symptoms and relieve the increase of muscle tension after cerebral hemorrhage in rats.Up-regulating GluR2 expression in in cervical enlargement of spinal cord may be one of the mechanisms by which electroacupuncture alleviates limb spasm after intracerebral hemorrhage.
作者
任慧玲
卢旭东
赵庆林
赵大巍
郭荣彬
李常法
步玮
REN Huiling;LU Xudong;ZHAO Qinglin;ZHAO Dawei;GUO Rongbin;LI Changfa;BU Wei(The Third Hospital of Hebei Medical University,Shijiazhuang 050051,Hebei,China)
出处
《现代中西医结合杂志》
CAS
2024年第3期316-321,共6页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
河北省中医药管理局科研计划项目(2020184)。
关键词
电针
脑出血
偏瘫
痉挛
脊髓
谷氨酸受体2
electro-acupuncture
cerebral hemorrhage
hemiplegia
spasm
spinal cord
glutamate receptor 2
