摘要
目的探讨利伐沙班(RIV)对氧化型低密度脂蛋白(Ox-LDL)诱导的人脐静脉内皮细胞(HUVEC)功能损伤的影响。方法根据干预措施,将实验分为空白对照组(A组,常规培养液),Ox-LDL组(B组,100μg/mL Ox-LDL的培养液),Ox-LDL+RIV125组(C1组,100μg/mL Ox-LDL和125 ng/mL RIV的培养液),Ox-LDL+RIV250组(C2组,100μg/mL Ox-LDL和250 ng/mL RIV的培养液)和Ox-LDL+RIV500组(C3组,100μg/mL Ox-LDL和500 ng/mLRIV的培养液),各组细胞均处理48 h。采用CCK-8法检测各组细胞存活率的变化,采用流式细胞术检测细胞凋亡情况,采用酶联免疫吸附试验(ELISA)检测白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,采用免疫印迹(Western blot)法检测细胞核因子-κB(NF-κB)和尿激酶型纤溶酶原激活因子受体(uPAR)蛋白表达水平。结果与B组比较,C1组、C2组、C3组细胞存活率显著升高(P<0.05),细胞凋亡率和uPAR表达水平均显著降低(P<0.05);C3组IL-1β,TNF-α,IL-6水平和p-NF-κB p65表达水平显著降低(P<0.05)。结论RIV能显著恢复细胞活力、减少细胞凋亡和由Ox-LDL引起的炎性反应,可能通过NF-κB和uPAR的调节而发挥作用。
Objective To investigate the effect of rivaroxaban(RIV)on oxidative low-density lipoprotein(Ox-LDL)-induced functional impairment of human umbilical vein endothelial cells(HUVEC).Methods According to different treatments,cells were divided into the blank control group(group A,conventional culture medium),Ox-LDL group(group B,culture medium containing 100μg/mL Ox-LDL),Ox-LDL+RIV125 group(group C1,culture medium containing 100μg/mL Ox-LDL and 125 ng/mL RIV),Ox-LDL+RIV250 group(group C_(2),culture medium containing 100μg/mL Ox-LDL and 250 ng/mL RIV)and Ox-LDL+RIV500 group(group C3,culture medium containing 100μg/mL Ox-LDL and 500 ng/mL RIV).Cells in all groups were treated for 48 h.CCK-8 method was used to detect the changes in cell survival in different groups,flow cytometry was used to detect apoptosis,enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α),and Western blot was used to determine the expression levels of nuclear factor-κB(NF-κB)and urokinase-type plasminogen activator receptor(uPAR)proteins.Results Compared with those in group B,the cell survival rates in groups C_(1),C_(2),and C_(3) significantly increased(P<0.05),while the cell apoptosis rates and the expression level of uPAR in groups C_(1),C_(2),and C_(3) significantly reduced(P<0.05);the levels of IL-1β,TNF-α,IL-6,and the expression level of p-NF-κB p65 in the group C_(3) significantly reduced(P<0.05).Conclusion RIV can significantly restore cell viability,reduce cell apoptosis,and induce inflammatory reactions induced by Ox-LDL,which may play a role through the regulation of NF-κB and uPAR.
作者
袁媛
刘迟
李光慧
张晓丹
王利苹
黄仲义
王斌
YUAN Yuan;LIU Chi;LI Guanghui;ZHANG Xiaodan;WANG Liping;HUANG Zhongyi;WANG Bin(Jing'an District Center Hospital,Shanghai,China 200040;Huashan Hospital of Fudan University,Shanghai,China 200040)
出处
《中国药业》
CAS
2024年第7期31-36,共6页
China Pharmaceuticals
基金
上海市静安区医学科研课题[2018QN02]
上海市静安区卫生系统后备人才计划项目[静卫健发〔2020〕30号]。
