摘要
研究了长白山地区著名野生食用菌元蘑的多糖组分的体外活性,通过水提醇沉法提取出元蘑多糖(HSP)经石油醚和Sevag试剂除杂后,通过DEAE纤维素-52离子交换层析和Sephadex G-100凝胶层析纯化,并测定了分离纯化后的组分对α-淀粉酶、胆固醇酯酶的抑制率、与胆酸盐的结合量。并通过线虫模型测定了元蘑多糖的抗衰老生物活性。在分离纯化试验中,由纯水、0.1、0.2 mol/L NaCl分别洗脱出了HSP-Ⅰ、HSP-Ⅱ、HSP-Ⅲ,再经凝胶层析后得到HSP-Ⅰ-1、HSP-Ⅱ-1。在活性试验中,HSP-Ⅰ-1、HSP-Ⅱ-1对α-淀粉酶的抑制率最高分别为1.48%、2.16%;对胆固醇酯酶的抑制率最高分别为34.26%、30.24%,抑制率与多糖质量浓度呈正相关,与石榴皮多糖和皂角米多糖相比具有较大的优势;与胆酸钠、甘胆酸钠、牛磺胆酸钠的结合量(m/m)平均分别为3.81%、3.40%;经过0.5、1.0 mg/mL元蘑多糖干预后的线虫平均寿命分别延长了8.6%、21.6%,且未对线虫产生生殖毒性。由此可知,元蘑多糖具有一定的降血脂和抗衰老活性,能够为元蘑的进一步开发提供理论依据。
This research investigated the in vitro activity of Hohenbuehelia serotina polysaccharides(HSP).HSP was obtained by water extraction and alcohol precipitation,treated with petroleum ether and Sevag and purified by DEAE cellulose-52 ion exchange chromatography and Sephadex G-100 gel chromatography.The inhibition rate ofα-amylase and cholesterol esterase and the binding amount of bile salt were investigated.The anti-aging activity of polysaccharides was detected in Caenorhabditis elegans.HSP-Ⅰ,HSP-Ⅱand HSP-Ⅲwere washed out by pure water,0.1 mol/L NaCl and 0.2 mol/L NaCl respectively.HSP-Ⅰ-1 and HSP-Ⅱ-1 were obtained after further purification by Sephadex G-100 gel chromatography.In the activity test,the inhibition effect onα-amylase of HSP-Ⅰ-1 and HSP-Ⅱ-1 was 1.48%and 2.16%.The highest inhibition rate of cholesterol esterase was 34.26%and 30.24%,and was positively related to the mass concentration of polysaccharides.Compared to polysaccharides from pomegranate peel and Gleditsia sinensis seeds,HSP had great advantage.The average combined amount of sodium cholate,sodium glycholate and sodium taurocholate of HSP-Ⅰ-1 and HSP-Ⅱ-1 was 3.81%and 3.40%(m/m).After treated with 0.5 mg/mL and 1.0 mg/mL polysaccharides,the average life span of C.elegans was increased by 8.6%and 21.6%respectively.There was no reproductive toxicity to C.elegans.The results showed that HSP had potent hypolipidemic and anti-aging activity.This research provided a theoretical foundation for the further development of HSP.
作者
李萍
徐伟烨
彭喜春
LI Ping;XU Wei-ye;PENG Xi-chun(Guangxi Vocational and Technical Institute of Industry,Nanning 530001,China;Jinan University,Guangzhou 510632,China)
出处
《江西农业学报》
CAS
2024年第1期63-68,共6页
Acta Agriculturae Jiangxi
基金
国家自然科学基金面上项目(32072223)
广西高等学校千名中青年骨干教师培育计划(桂教教师[2020]58号)。
关键词
元蘑多糖
降糖
降脂
抗衰老
Hohenbuehelia serotina polysaccharide
Hypoglycemic
Hypolipidemic
Anti-aging
