摘要
通过制备松花粉水提物、水提大分子化合物(>5 kDa)、乙醇提物和乙醇提小分子化合物(<5 kDa),测定各组提取物中的总糖、粗多糖、总酚和总黄酮含量,并考察其对摄入环磷酰胺SD大鼠卵巢的保护作用。结果表明,相对于模型组,松花粉乙醇提物及乙醇提小分子化合物组有效提升了卵巢指数、成熟卵泡数量、卵巢抗氧化酶活力(总抗氧化能力、过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶活力分别提升了16.03%、25.81%,138.42%、237.09%,104.31%、109.90%和91.62%、120.63%)和抗细胞凋亡蛋白表达,抑制闭锁卵泡和卵巢脂质氧化(丙二醛分别降低了53.42%和48.43%)等,效果优于松花粉水提物及水提大分子化合物组,但松花粉水提物及水提大分子化合物可以通过降低促炎因子肿瘤坏死因子-α活性保护卵巢。本研究可为进一步探明松花粉的卵巢保护作用机制提供理论基础,为开发基于松花粉提取物的新产品提供技术指导。
In this study,the contents of total sugars,crude polysaccharides,total phenols and total flavonoids in the aqueous and ethanol extracts of pine pollen,the large molecules isolated from the aqueous extract(>5 kDa),and the small molecules isolated from the ethanol extract(<5 kDa)were determined,and their protective effects on ovarian injury induced by cyclophosphamide in SD rats were explored.The results showed that compared with the model group,the ethanol extract and its small molecule fraction effectively increased the ovarian index,the number of mature follicles,the ovarian antioxidant enzyme activities total antioxidant capacity(T-AOC),catalase(CAT),glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)(by 16.03%,25.81%;138.42%,237.09%;104.31%,109.90%;and 91.62%,120.63%,respectively),and anti-apoptotic protein expression.In addition,they inhibited atretic follicles and ovarian lipid oxidation,as evidenced by a decrease in the content of malondialdehyde(MDA)by 53.42%and 48.43%,respectively;the effect was more pronounced than that of the aqueous extract and its large molecule fraction,while the aqueous extract and its large molecule fraction could protect the ovaries by reducing the pro-inflammatory cytokine tumor necrosis factor-α(TNF-α)activity.This study provides a theoretical basis for further investigation of the mechanism of the ovario-protective effect of pine pollen and also provides technical guidance for the development of new products based on pine pollen extracts.
作者
刘其耸
陈阳
杨长军
何毓婕
陈秋铭
何志勇
王召君
陈洁
曾茂茂
LIU Qisong;CHEN Yang;YANG Changjun;HE Yujie;CHEN Qiuming;HE Zhiyong;WANG Zhaojun;CHEN Jie;ZENG Maomao(College of Food and Biosystem Engineering,Yantai Institute of Technology,Yantai 264000,China;Yantai New Era Health Industry Co.Ltd.,Yantai 264006,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第6期105-112,共8页
Food Science
基金
国家食品科学与工程一流学科建设项目(JUFSTR20180201)。
关键词
松花粉
水提物
乙醇提物
水提大分子化合物
乙醇提小分子化合物
卵巢损伤
pine pollen
aqueous extracts
alcoholic extracts
aqueous soluble large molecules
ethanol soluble small molecules
ovarian injury
