摘要
为了解重唇石斛(Dendrobium hercoglossum)的化学成分,利用硅胶、Sephadex LH-20柱色谱和半制备高效液相色谱等方法,从其地上部分分离了14个化合物,经波谱分析,其结构分别鉴定为:3,4,5-三甲氧基苯酚(1)、3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-propan-1-one(2)、methyl 3-(p-hydroxyphenyl)propionate(3)、justiciresinol(4)、榕醛(5)、异落叶松脂素(6)、threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol(7)、(+)-松脂素(8)、aloifolⅠ(9)、dendrocandin T(10)、3-甲氧基-4-羟基苯丙酮(11)、1,3,5-三甲氧基苯酚(12)、香草醇(13)和丁香脂素(14),其中化合物1~12,14均为首次从重唇石斛中分离得到,化合物7、10和14为首次从石斛属植物中分离得到。化合物4、8和10具有显著的酪氨酸酶抑制活性,IC50值分别为(10.40±0.36)、(12.16±0.18)和(9.46±0.78)μmol/L。
In order to understand the chemical constituents of Dendrobium hercoglossum,fourteen compounds were isolated from the aerial parts of D.hercoglossum by using various column chromatography with silica gel,sephadex LH-20,and semi-preparative HPLC.Based on spectroscopic data,their structures were identified as 3,4,5-trimethoxyphenol(1),3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-propan-1-one(2),methyl 3-(p-hydro-xyphenyl)propionate(3),justiciresinol(4),ficusal(5),meso-secoisolariciresinol(6),threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol(7),(+)-pinoresinol(8),aloifolⅠ(9),dendrocandins T(10),3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-propan-1-one(11),1,3,5-trimethoxybenzene(12),homovanillyl alcohol(13)and(−)-syringaresinol(14).Compounds 1−12,14 were obtained from D.hercoglossum for the first time,and compounds 7,10,and 14 were isolated from Dendrobium species for the first time.The compounds 4,8 and 10 showed significant inhibitory activity,with IC50 of(10.40±0.36),(12.16±0.18)and(9.46±0.78)μmol/L,respectively.
作者
郭东昇
杨理
宋希强
梅文莉
陈惠琴
戴好富
GUO Dongsheng;YANG Li;SONG Xiqiang;MEI Wenli;CHEN Huiqin;DAI Haofu(Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants,Ministry of Education,School of Forestry,Hainan University,Haikou 570228,China;Key Laboratory of Natural Products Research and Development of Li Folk Medicine of Hainan Province Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences&Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province,Hainan Institute for Tropical Agricultural Resources,Haikou 571101,China)
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2024年第2期287-293,共7页
Journal of Tropical and Subtropical Botany
基金
海南省自然科学基金项目(320QN337)
海南省院士创新平台科研项目资助。
关键词
兰科
重唇石斛
化学成分
酪氨酸酶
Orchidacae
Dendrobium hercoglossum
Chemical constituent
Tyrosinase
