摘要
目的探讨秦皮乙素(AES)对胃癌SGC-7901细胞增殖、迁移、侵袭和糖酵解的影响及相关机制。方法采用MTT法检测细胞增殖活性;采用划痕实验和Transwell实验检测细胞迁移和侵袭能力;葡萄糖摄取量测定和乳酸含量测定实验检测细胞糖酵解情况;Western blot法检测迁移、侵袭及糖酵解相关蛋白的表达水平。结果AES能够抑制SGC-7901细胞的增殖活力,且这种抑制效果与AES的剂量成正相关。随着AES剂量的梯度增加,SGC-7901细胞的迁移、侵袭及糖酵解能力逐渐降低(P<0.05)。AES可以下调SGC-7901细胞HIF-1α、MMP-2、MMP-9、GLUT1、LDHA蛋白的表达水平(P<0.05)。结论AES对人胃癌SGC-7901细胞增殖活性及迁移、侵袭能力有抑制作用,通过抑制人胃癌SGC-7901细胞的葡萄糖摄取及乳酸生成降低糖酵解水平。AES可能通过影响缺氧诱导因子HIF-1α抑制SGC-7901细胞迁移、侵袭及有氧糖酵解过程。
Objective To determine the effect and mechanism of aesculetin(AES)on the proliferation,migration,invasion and glycolysis of human gastric cancer SGC-7901 cells.Methods The cell proliferation was detected by MTT assay.The cell migration and invasion were detected by scratch test and transwell test.Glucose uptake test and lactic acid content test were used to detect the glycolysis of cells.The expression level of migration,invasion and glycolytic related proteins were detected by Western blot.Results AES inhibited the proliferation of SGC-7901 cells in a dosedependent manner.The migration,invasion and glycolysis of SGC-7901 cells decreased gradually with the gradient increase of AES doses(P<0.05).AES down-regulated the protein expression of HIF-1α,MMP-2,MMP-9,GLUT1 and LDHA in SGC-7901 cells(P<0.05).Conclusion AES can inhibit the proliferation,migration and invasion of human gastric cancer SGC-7901 cells,and reduce the glycolysis level by inhibiting the glucose uptake and lactic acid production of human gastric cancer SGC-7901 cells.AES may inhibit the migration,invasion and aerobic glycolysis of SGC-7901 cells by influencing hypoxia-inducing factor HIF-1α.
作者
贾绍华
郭浩
丁海鑫
孙萌遥
JIA Shao-hua;GUO Hao;DING Hai-xin;SUN Meng-yao(School of Pharmacy(Engineering Research Center for Medicine),Harbin University of Commerce,Harbin 150076)
出处
《中南药学》
CAS
2024年第3期679-684,共6页
Central South Pharmacy
基金
黑龙江省自然科学基金重点项目(No.ZD2018016)。
