摘要
Plasma-activated water(PAW),as an extended form of cold atmospheric-pressure plasma,greatly expands the application of plasma-based technology.The biological effects of PAW are closely related to the aqueous reactive species,which can be regulated by the activation process.In this study,surface plasma-activated air(SAA)and a He+O_(2)plasma jet(Jet)were parallelly combined(the SAA+Jet combination)or sequentially combined(the SAA→Jet combination and the Jet→SAA combination)to prepare plasma-activated saline(PAS).The PAS activated by the combinations exhibited stronger bactericidal effects than that activated by the SAA or the Jet alone.The concentrations of H_(2)O_(2)and NO_(2)^(-)were higher in the PAS activated by the Jet→SAA combination,while ONOO^(-)concentrations were close in the three kinds of PAS and^(1)O_(2)concentrations were higher in the PAS activated by the SAA+Jet combination.The analysis of scavengers also demonstrated that H_(2)O_(2),^(1)O_(2),and ONOO^(-)in the PAS activated by the SAA+Jet combination,and^(1)O_(2)in the PAS activated by the Jet→SAA combination played critical roles in bactericidal effects.Further,the effective placement time of the three PAS varied,and the PAS activated by the Jet→SAA combination could also inactivate 2.6-log_(10)of MRSA cells after placement for more than 60 min.The regulation of reactive species in plasma-activated water via different combinations of plasma devices could improve the directional application of plasma-activated water in the biomedical field.
作者
贾怡康
李甜会
张瑞
赵鹏瑜
王子丰
陈旻
郭莉
刘定新
Yikang JIA;Tianhui LI;Rui ZHANG;Pengyu ZHAO;Zifeng WANG;Min CHEN;Li GUO;Dingxin LIU(State Key Laboratory of Electrical Insulation and Power Equipment,Center for Plasma Biomedicine,Xi’an Jiaotong University,Xi’an 710049,People’s Republic of China;School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049,People’s Republic of China)
基金
supported by National Natural Science Foundation of China(No.51977174)。
