摘要
再植障碍问题限制了当归产业健康发展。为了建立高效种植方式,在甘肃渭源县设置5种种植方式[A:豌豆-小麦-当归;B:豌豆-蒙古黄芪-当归;C:豌豆-马铃薯-当归;D:豌豆-当归-当归(对照);E:豌豆-休耕-当归],采挖期通过Illumina Hisqe 2500高通量测序平台测定了不同种植模式下当归根际土壤真菌ITS1变异区的基因序列,分析不同种植方式对当归根际土壤真菌群落和功能差异性的影响。结果表明:(1)5种种植方式下当归根际真菌多样性差异不大,较对照D处理,A、B、C和E处理的Chao1指数、Ace指数和Shannon指数较低,Simpson指数较高。(2)5种种植方式下当归根际土壤真菌群落隶属于11门167属,其中,子囊菌门(Ascomycota)、被孢菌门(Mortierellomycota)、担子菌亚门(Basidiomycota)和壶菌门(Chytridiomycota)为优势门;被孢霉属(Mortierella)、四枝孢属(Tetracladium)为优势属。(3)冗余度分析发现:在门水平影响土壤真菌群落结构组成的主要因子为速效钾、电导率、pH值,在属水平影响土壤真菌群落结构组成的主要因子为有机质、pH值、二氧化碳呼吸速率。(4)FUNGuild预测表明:植物病原体和木质腐生真菌功能群的相对丰度较高。综上所述,不同作物轮作较当归连作,降低了当归根际土壤真菌群落的相对丰度和多样性,有利于根际土壤真菌群落向有益的方向发展。
The problem of replanting obstacle limits the healthy development of Angelica sinensis industry.In order to establish an efficient planting method,five planting methods were established in Weiyuan county,Gansu province,including A:pea-wheat-A.sinensis;B:pea-Mongolian astragalus-A.sinensis;C:pea-potato-A.sinensis;D:pea-A.sinensis-A.sinensis(control);E:pea-fallow-A.sinensis.The gene sequences of ITS1 variation region of fungi in the rhizosphere soil under different cropping patterns were determined during A.sinensis harvest period by Illumina Hisqe 2500 sequencing platform,and the effects of different cropping patterns on fungal communities and functional differences in the rhizosphere soil were analyzed.The results showed as follows:(1)Compared to the control D,the Chao1 index,Ace index and Shannon index for A,B,C and E treatments were lower,but the Simpson index was higher.(2)Soil fungi communities in the rhizosphere under five planting methods attached to 167 genera in 11 phyla,in which Ascomycota,Mortierellomycota,Basidiomycota and Chytridiomycota were the dominant phyla and Mortierella and Tetracladium were the dominant genera.(3)Redundancy analysis showed that available potassium,electrical conductivity,and pH value were the main factors affecting soil fungal community structure at phylum level,while organic matter,pH value,and carbon dioxide respiration rate were the main factors affecting soil fungal community structure at genus level.(4)FUNGuild prediction showed that the relative abundances of functional groups of plant pathogens and woody saprophytic fungi were higher.In conclusion,compared with continuous cropping A.sinensis,different crops rotation reduced the relative abundance and diversity of fungal communities in the rhizosphere soil,which was conducive to the profitable development of soil fungal communities in the rhizosphere soil.
作者
姜小凤
郭凤霞
陈垣
郭建国
JIANG Xiao-feng;GUO Feng-xia;CHEN Yuan;GUO Jian-guo(Dryland Agriculture Institute/Institude of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070;Key Laboratory of High Water Utilization on Drvland of Gansu Province,Lanzhou Gansu 730070;College of Life Science and Technology/Agronomy College,Gansu Agricultural University,Lanzhou Gansu 730070)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2024年第1期28-36,共9页
Soil and Fertilizer Sciences in China
基金
甘肃省科技计划项目(民生科技专项-乡村振兴专题22CX2NA007)
甘肃省农业科学院农业科技自主创新专项重点研发计划项目(2021 GARS 11)
现代丝路寒旱农业科技支撑项目(GSLK-2022-6)
国家重点研发计划课题及子课题(2021YFD1100504
2021YFD11005041
2021YFD11005042)
国家自然科学基金(31460547,31560175,31360317)。
关键词
土壤真菌
真菌群落
功能群
轮作
连作
soil fungi
soil fungal community
functional groups
crop rotation
continuous cropping
