摘要
目的:建立了采用高效液相色谱-高分辨率四级杆-飞行时间质谱(HPLC-Q-TOP-MS)法,检测祛痘类化妆品中20种非法添加抗感染类药物的方法。方法:采用Agilent EC-C18色谱柱(3.0×150 mm,2.7μm);流动相为乙腈-0.1%冰醋酸溶液,梯度洗脱;流速为0.3 mL/min;柱温为35℃;电喷雾电离源,正离子模式,根据MassHunter软件提取的保留时间、母离子、碎片离子精确质量等数据,在PCDL软件中构建了非法添加抗感染类药物的筛查数据库。结果:氯霉素和螺内酯在质量浓度100~5000ng/mL范围内,其余18种抗感染药物在质量浓度50~5000 ng/mL范围内线性关系良好(r>0.99),检出限为0.01~0.20μg/g,定量限为0.05~0.80μg/g,回收率为83.7%~108.8%,相对标准偏差(RSD)为1.2%~8.1%。结论:本法操作简单、准确可靠,适用于祛痘类化妆品中20种抗感染药物的快速筛查,为祛痘类化妆品的安全监管提供了强有力的技术支撑。
Objective:To establish a method for detecting 20 illegal anti-infective drugs in acne cosmetics by HPLC-Q-TOP-MS.Methods:An Agilent EC-C18 column(3.0×150 mm,2.7μm)was adopted at the flow rate of 0.3 mL/min with the mobile phase was acetonitrile-0.1%glacial acetic acid solution,and the gradient eluted;The column temperature is 35℃;Positive ion mode electrospray ionization,based on the retention time,precursor ion,fragment ion accurate mass and other data extracted by MassHunter,a screening database for illegally added anti-infective drugs was constructed in PCDL.Results:Chloramphenicol and spironolactone showed a good linear relationship(r>0.99)in the range of mass concentration of 100~5000 ng/mL,while the remaining 18 anti-infective drugs showed a good linear relationship in the range of mass concentration of 50~5000 ng/mL.The detection limit was 0.01~0.20μg/g,the quantification limit was 0.05~0.80μg/g,the recovery rate was 83.7%~108.8%,and the relative standard deviation(RSD)was 1.2%~8.1%.Conclusion:This method is simple,accurate and reliable,and is suitable for the rapid screening of 20 anti-infective drugs in acne cosmetics,which provides strong technical support for the safety supervision of acne cosmetics.
作者
郑篮君
舒展
王琤帅
凌明
滕云龙
Zheng Lanjun;Shu Zhan;Wang Chengshuai;Ling Ming;Teng Yunong(Jinhua Institute for Drug and Food Control,Jinhua 321000,China)
出处
《广东化工》
CAS
2024年第4期125-128,共4页
Guangdong Chemical Industry
基金
金华市科技计划项目(2020-4-101)。
