摘要
为了探究外源BtCry1Ac基因对107杨材性性状产生的非预期影响,以转BtCry1Ac基因107杨2个株系(ECA1和ECA2)和野生型株系(WT)为材料,对其木材化学组分、物理和力学性质及纤维形态指标进行了定量测定。结果显示:BtCry1Ac基因的转入使107杨木材材性发生了非预期变化,具体表现为ECA1和ECA2的纤维素、灰分、苯醇抽出物及1%NaOH抽出物组分含量均显著低于WT(P<0.05);ECA1和ECA2的气干干缩性、全干干缩性显著低于WT(P<0.05),且ECA1和ECA2的气干密度、全干密度显著高于WT(P<0.05);ECA1和ECA2的冲击韧性、抗弯强度、抗弯弹性模量和顺纹抗压强度均显著高于WT(P<0.05);ECA1和ECA2的纤维长度显著低于WT(P<0.05)。总体表现为转基因株系的木材有着更小的干缩性,更高的密度和强度,其材性发生了非预期性改变,研究结果为转基因杨树的推广与应用提供了参考。
The purpose of this study was to explore the unexpected effects of exogenous BtCry1Ac gene on wood properties of Populus×euramericana‘74/76’(poplar 107).The wild type(WT)and two overexpression BtCry1Ac lines(ECA1 and ECA2)of poplar 107 were selected,and their stems were used as materials.The chemical composition,physical properties,mechanical properties,and fiber morphology of the stems were quantitatively measured.The results demonstrated that heterologous expression of BtCry1Ac caused an unexpected variation of the timber properties(i.e.,enhanced the timber’s mechanical strength and drying shrinkage)in poplar 107.In details,the contents of the cellulose,ash,benzene alcohol extract,and 1%(v/v)NaOH extract of the ECA1 and ECA2 lines were lower(P<0.05)than those of the WT line.The indexes of air/total dry shrinkage of the two transgenic lines were lower(P<0.05);and the air/total dry densities were higher than those of the WT line.Furthermore,the impact toughness,flexural strength,flexural elastic modulus,and cis-grain compressive strength in the two OE-BtCry1Ac lines were both higher(P<0.05)compared to the WT line.Moreover,the fiber lengths of the two OE-BtCry1Ac lines were both higher than those of the WT line(P<0.05).As a whole,the wood of transgenic lines has less drying shrinkage,higher density and strength,and its wood properties have changed unexpectedly.The results provide a reference for the popularization and application of transgenic poplar.
作者
李洪瑞
赵志明
张津
门艳阔
张子杨
王进茂
LI Hongrui;ZHAO Zhiming;ZHANG Jin;MEN Yankuo;ZHANG Ziyang;WANG Jinmao(College of Forestry,Hebei Agricultural University,Baoding Hebei 071000,P.R.China;Hebei Hongyashan State Forestry Farms,Yixian Hebei 074200,P.R.China)
出处
《西部林业科学》
CAS
北大核心
2024年第1期82-87,共6页
Journal of West China Forestry Science
基金
河北重点研发计划(21326301D)。
