摘要
该研究以分离自豆豉样品中产豆豉纤溶酶的枯草芽孢杆菌(Bacillus subtilis)DC-1为出发菌株,采用紫外线诱变选育高产豆豉纤溶酶且稳定遗传的菌株,并以豆豉纤溶酶活力为评价指标,通过单因素试验及正交试验对其发酵条件进行优化。结果表明,筛选出一株高产豆豉纤溶酶活力且稳定遗传的诱变菌株DC-V5,其最优发酵条件为:接种量3%、装液量70 mL/250 mL、发酵温度34℃、初始pH 6.5。在此优化发酵条件下,诱变菌株DC-V5产豆豉纤溶酶活力最高达到(451.26±11.09)IU/mL,是优化前出发菌株DC-1的1.74倍。
Using Douchi fibrinolytic enzyme-producing strain Bacillus subtilis DC-1 isolated from Douchi samples as the original strain,the high-yield Douchi fibrinolytic enzyme strain with stable inheritance was bred by ultraviolet mutagenesis,and the fermentation conditions were optimized by single factor tests and orthogonal tests using Douchi fibrinolytic enzyme activity as evaluation index.The results showed that a mutagenic strain DC-V5 with high Douchi fibrinolytic enzyme activity and genetic stability was obtained,and the optimal fermentation conditions were as follows:inoculum 3%,liquid loading volume 70 ml/250 ml,fermentation temperature 34℃and initial pH 6.5.Under the optimal fermentation conditions,the Douchi fibrinolytic enzyme activity of strain DC-V5 reached(451.26±11.09)IU/ml,which was 1.74 times that of the original strain DC-1.
作者
张文勇
张恒慧
王晓丽
ZHANG Wenyong;ZHANG Henghui;WANG Xiaoli(Department of Environmental and Safety Engineering,Taiyuan Institute of Technology,Taiyuan 030008,China)
出处
《中国酿造》
CAS
北大核心
2024年第3期177-181,共5页
China Brewing
基金
大学生创新项目(S202314101143,GK2023096)。
关键词
枯草芽孢杆菌
豆豉纤溶酶
紫外线诱变
产酶条件优化
Bacillus subtilis
Douchi fibrinolytic enzyme
ultraviolet mutagenesis
enzyme-producing condition optimization
