摘要
【目的】通过体细胞克隆技术生产超细型二狼山绒山羊克隆个体,为该技术在地方优质羊品种资源扩繁及新品种培育中的应用提供理论依据。【方法】选择1只4岁龄羊绒细度为13.89μm的二狼山绒山羊(♂),采集耳组织并分离培养成纤维细胞,以其为供体细胞,利用体细胞克隆技术开展超细型二狼山绒山羊的扩繁研究,通过克隆羊体重、羊绒细度、繁殖性能等指标评估该技术的可行性。【结果】试验成功建立了稳定的山羊卵母细胞体外成熟培养及体细胞克隆技术体系,卵母细胞成熟率为44.8%,孤雌激活卵裂率、囊胚率分别为83.0%、22.4%;二狼山绒山羊克隆胚的卵裂率、囊胚率分别为77.8%、13.7%。获得5只克隆羊个体,其生长发育正常,二狼山绒山羊母羊自然交配共获得16只健康的F1代个体,具有正常繁殖能力。克隆个体羊绒细度极显著低于同龄对照个体(P<0.01)。【结论】本研究通过体细胞克隆技术成功克隆了超细型二狼山绒山羊个体,其具备正常生产性能和繁殖性能,并保留了超细羊绒的特性,为地方优良羊种质资源扩繁和新品种培育应用研究奠定了基础。
【Objective】The cloned individual of superfine-type Erlangshan cashmere goat was produced by somatic cell cloning technology,which provided a theoretical basis for the application of this technology in the expansion of local high-quality sheep breed resources and the breeding of new varieties.【Method】A 4-year old Erlangshan cashmere goat with a cashmere fineness of 13.89μm was selected.The ear tissue was collected and cultured as fibroblasts,which were used as the donor cells.Somatic cell cloning technology was used to expand the propagation of superfine-type Erlangshan cashmere goat.The feasibility of the technique was evaluated by weight,fineness of cashmere and reproductive performance of cloned sheep.【Result】The stable system of goat oocyte maturation culture in vitro and somatic cell cloning was successfully established.The oocyte maturation rate was 44.8%,and the cleavage rate and blastocyst rate of parthenogenesis was 83.0%and 22.4%,respectively.The cleavage rate and blastocyst rate of the cloned embryos of Erlangshan cashmere goat was 77.8%and 13.7%,respectively.5 cloned sheep were obtained with normal growth and development,and 16 healthy F1 generation individuals were obtained by natural mating with Erlangshan ewes,with normal reproductive ability.The cashmere fineness of cloned individuals was significantly lower than that of control individuals of the same age(P<0.01).【Conclusion】Superfine-type Erlangshan cashmere goat individuals was successfully cloned by somatic cell cloning technology,which had normal production and reproductive performance,and retained the characteristics of superfine cashmere,laid a foundation for the expansion of native excellent sheep germplasm resources and the application of new varieties breeding.
作者
卿玉波
程雯
角德灵
刘城
郝志强
郭建雄
李钰莹
张筱琳
李臻
李效宇
王文
魏红江
QING Yubo;CHENG Wen;JIAO Deling;LIU Cheng;HAO Zhiqiang;GUO Jianxiong;LI Yuying;ZHANG Xiaolin;LI Zhen;LI Xiaoyu;WANG Wen;WEI Hongjiang(Key Laboratory for Porcine Gene Editing and Xenotransplantation in Yunnan Province,Kunming 650201,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Inner Mongolia Zhongke Zhengbiao Biotechnology Co.,Ltd.,Bayannur 015000,China;Inner Mongolia Zhengbiao Breeding Stock Co.,Ltd.,Bayannur 015000,China;Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650201,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2024年第4期1420-1427,共8页
China Animal Husbandry & Veterinary Medicine
基金
云南省国际科技合作专项-巴基斯坦肉羊扩繁技术集成、应用与推广(2016IA024)。
关键词
二狼山绒山羊
体细胞克隆
羊绒细度
繁殖性能
Erlangshan cashmere goat
somatic cell cloning
cashmere fineness
reproductive performance
