摘要
牛病毒性腹泻病毒(BVDV)是危害养牛业的重要病原。2021年本实验室从患有肺炎的牛肺中分离出1株BVDV(毒株编号为JS2201),经鉴定为1b亚型。为了评价该分离株的毒力与致病性,分别建立了分离株与参考株BVDV-1型Oregon C24V株、BVDV-2型C1602株BVDV感染BALB/c小鼠模型,通过检测攻毒后小鼠临床症状、体重变化、病毒血症情况、血液中白细胞与淋巴细胞的变化、组织器官的病理组织学变化及病毒载量,比较了分离株JS2201与1型和2型参考株的致病性差异。结果显示:BVDV分离株JS2201与参考株均能够成功感染BALB/c小鼠,攻毒BALB/c小鼠均形成病毒血症,导致血液中白细胞数量、淋巴细胞数量以及淋巴细胞百分比短暂下降;BVDV分离株JS2201株攻毒组小鼠肝脏、肾脏、肺脏、脾脏中BVDV载量要高于C24V、C1602株攻毒组;攻毒组小鼠均表现为间质性肺炎病变,JS2201株攻毒组与1型参考株C24V株攻毒组小鼠肺脏病理组织学变化相似,BVDV-2型参考株C1602株攻毒组小鼠肺脏病变更为严重。综上,本研究成功建立了不同亚型BVDV急性感染BALB/c小鼠的模型,且比较分析了分离株与参考株之间的致病性差异,为BVDV疫苗的研制以及致病机制的研究奠定了基础。
Bovine viral diarrhea virus(BVDV)is an important pathogen that endangers the cattle industry,and it causes huge economic losses to the world's cattle industry.With the increase of international trade,the cattle have been transported frequently,BVDV strains con⁃tinue to mutate,and new gene subtypes continue to appear.In 2021,our laboratory isolated a BVDV(JS2201)from bovine lung with pneu⁃monia.In order to evaluate the virulence and pathogenicity of the BVDV isolate in our laboratory,we established respective BALB/c mice models infected with the BVDV isolated strain,the BVDV-1 type Oregon C24V reference strain,and BVDV-2 type C1602.By observing and detecting the clinical symptoms,weight changes,viremia,changes in white blood cells and lymphocytes in different groups of mice after challenge,the histopathological changes and viral load of various tissues and organs were compared.The results showed that both the BVDV isolate JS2201 and the reference strain were able to infect BALB/c mice successfully,and the infected BALB/c mice developed viremia,re⁃sulting in a temporary decrease in the number of white blood cells,lymphocytes and percentage of lymphocytes in the blood.The BVDV loads in the liver,kidney,lung and spleen of the mice infected with the BVDV isolate JS2201 strain were higher than those in the C24V and C1602 infected groups.All mice in the challenged group showed interstitial pneumonia.The pathological changes of lung in the challenged group of the JS2201 strain,and the reference strain C24V of type 1 strain were similar.The lung disease in the challenged group of the BVDV reference strain C1602 strain got more severe.In this study,the model of acute infection of BALB/c mice with different subtypes of BVDV was successfully established,and the pathogenicity difference between the isolated strain and the reference strain were compared and ana⁃lyzed;which laid the foundation for the development of BVDV vaccines and the study of their pathogenesis.
作者
刘镝钺
程子龙
陈益
陈思宇
李文良
毛立
杨蕾蕾
孙敏
张纹纹
熊富强
刘茂军
LIU Diyue;CHENG Zilong;CHEN Yi;CHEN Siyu;LI Wenliang;MAO Li;YANG Leilei;SUN Min;ZHANG Wenwen;XIONG Fuqiang;LIU Maojun(College of Animal Sciences,Tibet Agriculture and Animal Husbandry College,Linzhi 860000,China;Key Laboratory of Veterinary Bioproducts Engineering Technology,Department of Agriculture and Rural Affairs/Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Guotai Technology Innovation Center for Veterinary Bioproducts(Taizhou),Taizhou 225300,China)
出处
《畜牧与兽医》
CAS
北大核心
2024年第4期72-77,共6页
Animal Husbandry & Veterinary Medicine
基金
国家重点研发计划项目(2022YFD1800603)。
